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The reaction between ferrocytochrome c and hydrogen peroxide
The reaction between ferrocytochrome c and hydrogen peroxide has been studied to determine possible reaction mechanisms. This was done by investigating the effect of various physical factors, such as hydrogen ion concentration, ionic strength and temperature, on the reaction and by studying the kine...
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| Format: | Thesis |
| Language: | English |
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Department of Geological Sciences
2016
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| _version_ | 1867613344200916992 |
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| access_status_str | Open Access |
| author | Hulett, Leslie Graham |
| author2 | Ahrens, L H |
| author_browse | Ahrens, L H Hulett, Leslie Graham |
| author_facet | Ahrens, L H Hulett, Leslie Graham |
| author_sort | Hulett, Leslie Graham |
| collection | Thesis |
| description | The reaction between ferrocytochrome c and hydrogen peroxide has been studied to determine possible reaction mechanisms. This was done by investigating the effect of various physical factors, such as hydrogen ion concentration, ionic strength and temperature, on the reaction and by studying the kinetics of the reaction. The study of pH and ionic strength showed that an ionization occurred at pH 7.8 which influenced the reactivity of the cytochrome c. The reaction was studied kinetically at pH 8.1 and pH 6.6. At pH 8.1 simple kinetics showed an uncomplicated reaction between ferrocytochrome c and hydrogen peroxide. The kinetic studies at pH 6.6 showed that ferrocytochrome c, hydrogen peroxide and ferricytochrome c (acting as a product catalyst) were involved in the reaction. The product catalysis complicated the kinetics and difficulty was experienced in fitting a suitable reaction mechanism. Copper ions have been found to catalyse reactions similar to that studied here. The effect of a copper histidine complex was therefore investigated on the reaction at pH 8.7. The copper complex was found to catalyse the oxidation of ferrocytochrome c by hydrogen peroxide and the kinetic studies of this oxidation showed that the reaction could be fitted to a simple mechanism which did not involve the formation of intermediate complexes. An unusual feature of the reaction was the ability of the cytochrome c, after complete oxidation, to undergo reduction, which is dependent on the presence of both hydrogen peroxide and copper ions. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/22300 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:39.078Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2016 |
| publishDateRange | 2016 |
| publishDateSort | 2016 |
| publisher | Department of Geological Sciences |
| publisherStr | Department of Geological Sciences |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/22300 The reaction between ferrocytochrome c and hydrogen peroxide Hulett, Leslie Graham Ahrens, L H Erlank, A J Geochemistry The reaction between ferrocytochrome c and hydrogen peroxide has been studied to determine possible reaction mechanisms. This was done by investigating the effect of various physical factors, such as hydrogen ion concentration, ionic strength and temperature, on the reaction and by studying the kinetics of the reaction. The study of pH and ionic strength showed that an ionization occurred at pH 7.8 which influenced the reactivity of the cytochrome c. The reaction was studied kinetically at pH 8.1 and pH 6.6. At pH 8.1 simple kinetics showed an uncomplicated reaction between ferrocytochrome c and hydrogen peroxide. The kinetic studies at pH 6.6 showed that ferrocytochrome c, hydrogen peroxide and ferricytochrome c (acting as a product catalyst) were involved in the reaction. The product catalysis complicated the kinetics and difficulty was experienced in fitting a suitable reaction mechanism. Copper ions have been found to catalyse reactions similar to that studied here. The effect of a copper histidine complex was therefore investigated on the reaction at pH 8.7. The copper complex was found to catalyse the oxidation of ferrocytochrome c by hydrogen peroxide and the kinetic studies of this oxidation showed that the reaction could be fitted to a simple mechanism which did not involve the formation of intermediate complexes. An unusual feature of the reaction was the ability of the cytochrome c, after complete oxidation, to undergo reduction, which is dependent on the presence of both hydrogen peroxide and copper ions. 2016-10-25T13:36:32Z 2016-10-25T13:36:32Z 1968 Master Thesis Masters MSc http://hdl.handle.net/11427/22300 eng application/pdf Department of Geological Sciences Faculty of Science University of Cape Town |
| spellingShingle | Geochemistry Hulett, Leslie Graham The reaction between ferrocytochrome c and hydrogen peroxide |
| thesis_degree_str | Master's |
| title | The reaction between ferrocytochrome c and hydrogen peroxide |
| title_full | The reaction between ferrocytochrome c and hydrogen peroxide |
| title_fullStr | The reaction between ferrocytochrome c and hydrogen peroxide |
| title_full_unstemmed | The reaction between ferrocytochrome c and hydrogen peroxide |
| title_short | The reaction between ferrocytochrome c and hydrogen peroxide |
| title_sort | reaction between ferrocytochrome c and hydrogen peroxide |
| topic | Geochemistry |
| url | http://hdl.handle.net/11427/22300 |
| work_keys_str_mv | AT hulettlesliegraham thereactionbetweenferrocytochromecandhydrogenperoxide AT hulettlesliegraham reactionbetweenferrocytochromecandhydrogenperoxide |