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Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum
Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutyli...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2017
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| _version_ | 1867614445688061952 |
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| access_status_str | Open Access |
| author | Youngleson, Jonathan Sinclair |
| author2 | Woods, David R |
| author_browse | Woods, David R Youngleson, Jonathan Sinclair |
| author_facet | Woods, David R Youngleson, Jonathan Sinclair |
| author_sort | Youngleson, Jonathan Sinclair |
| collection | Thesis |
| description | Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutylicum, which has as an ultimate goal the commercial improvement, and a fundamental understanding of the ABE fermentation. The aim of this study was to isolate and characterize genes involved in solventogenesis. The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/23245 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:52:09.802Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/23245 Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum Youngleson, Jonathan Sinclair Woods, David R Jones, David T Microbiology Clostridium acetobutylicum P262 is an endospore-forming Gram-positive anaerobic bacterium, which has been used for the industrial production of acetone and butanol from carbohydrate substrates. This study forms part of a wider research effort into the genetics and molecular biology of C. acetobutylicum, which has as an ultimate goal the commercial improvement, and a fundamental understanding of the ABE fermentation. The aim of this study was to isolate and characterize genes involved in solventogenesis. The cloning, expression and characterization of the terminal solventogenic butanol dehydrogenase gene ( adhl), and the central pathway β-hydroxybutyryl-CoA dehydrogenase gene (hbd), which form part of a but operon are described. 2017-01-26T07:34:12Z 2017-01-26T07:34:12Z 1989 2016-11-22T11:22:14Z Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/23245 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Youngleson, Jonathan Sinclair Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum |
| thesis_degree_str | Doctoral |
| title | Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum |
| title_full | Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum |
| title_fullStr | Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum |
| title_full_unstemmed | Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum |
| title_short | Molecular genetic characterization of two solvent pathway dehydrogenases from Clostridium Acetobutylicum |
| title_sort | molecular genetic characterization of two solvent pathway dehydrogenases from clostridium acetobutylicum |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/23245 |
| work_keys_str_mv | AT younglesonjonathansinclair moleculargeneticcharacterizationoftwosolventpathwaydehydrogenasesfromclostridiumacetobutylicum |