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Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions

This research pertains to bioleaching of copper containing ores with particular reference to the copper sulfide mineral chalcopyrite (CuFeS2). While it is focused on heap bioleaching, it has applications to stirred tank bioleaching operations. In the context of bioleaching, microbial extra-cellular...

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Main Author: Africa, Cindy-Jade
Other Authors: Harrison, Susan T L
Format: Thesis
Language:English
Published: Centre for Bioprocess Engineering Research 2017
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access_status_str Open Access
author Africa, Cindy-Jade
author2 Harrison, Susan T L
author_browse Africa, Cindy-Jade
Harrison, Susan T L
author_facet Harrison, Susan T L
Africa, Cindy-Jade
author_sort Africa, Cindy-Jade
collection Thesis
description This research pertains to bioleaching of copper containing ores with particular reference to the copper sulfide mineral chalcopyrite (CuFeS2). While it is focused on heap bioleaching, it has applications to stirred tank bioleaching operations. In the context of bioleaching, microbial extra-cellular polymeric substance (EPS) components are thought to complex chemical oxidants and extend the chemical reaction space available for mineral dissolution reactions, making the microbial-mineral-EPS interface the dominant active zone in terms of microbial oxidation and mineral dissolution. There is a limited understanding of microbial biofilm formation within a bioleach heap. The implication of various microorganisms having a set of defined or optimal conditions under which they colonise and proliferate is quite substantial. Understanding what creates favourable interfacial microenvironments enabling a sessile population to flourish (and thereby decrease lag time) has great implications for minimising costs and maximising productivity. Furthermore, limited work has been conducted on thermophilic microorganisms relevant to bioleaching. These microorganisms are pertinent to successful bioleaching at high temperatures, with work incorporating low grade ores and gangue mineralogy also being scarce. The aim of this research is to provide a thorough investigation into microbial-metal sulfide interfacial environments in situ, using a thermophilic archaeon M. hakonensis, low-grade metal-sulfide ores, a series of temperature regimes, heap-simulating conditions and an in depth extraction and analysis of the EPS produced under varied culturing conditions.
format Thesis
id oai:open.uct.ac.za:11427/25001
institution University of Cape Town (South Africa)
language eng
last_indexed 2026-06-10T12:33:43.673Z
license_str Not specified — see source repository
provenance_str_mv Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository
publishDate 2017
publishDateRange 2017
publishDateSort 2017
publisher Centre for Bioprocess Engineering Research
publisherStr Centre for Bioprocess Engineering Research
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source_str UCTD — University of Cape Town Open Access Repository
spelling oai:open.uct.ac.za:11427/25001 Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions Africa, Cindy-Jade Harrison, Susan T L Van Hille, Robert P Bioprocess Engineering This research pertains to bioleaching of copper containing ores with particular reference to the copper sulfide mineral chalcopyrite (CuFeS2). While it is focused on heap bioleaching, it has applications to stirred tank bioleaching operations. In the context of bioleaching, microbial extra-cellular polymeric substance (EPS) components are thought to complex chemical oxidants and extend the chemical reaction space available for mineral dissolution reactions, making the microbial-mineral-EPS interface the dominant active zone in terms of microbial oxidation and mineral dissolution. There is a limited understanding of microbial biofilm formation within a bioleach heap. The implication of various microorganisms having a set of defined or optimal conditions under which they colonise and proliferate is quite substantial. Understanding what creates favourable interfacial microenvironments enabling a sessile population to flourish (and thereby decrease lag time) has great implications for minimising costs and maximising productivity. Furthermore, limited work has been conducted on thermophilic microorganisms relevant to bioleaching. These microorganisms are pertinent to successful bioleaching at high temperatures, with work incorporating low grade ores and gangue mineralogy also being scarce. The aim of this research is to provide a thorough investigation into microbial-metal sulfide interfacial environments in situ, using a thermophilic archaeon M. hakonensis, low-grade metal-sulfide ores, a series of temperature regimes, heap-simulating conditions and an in depth extraction and analysis of the EPS produced under varied culturing conditions. 2017-08-28T13:14:54Z 2017-08-28T13:14:54Z 2017 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/25001 eng application/pdf Centre for Bioprocess Engineering Research Faculty of Engineering and the Built Environment University of Cape Town
spellingShingle Bioprocess Engineering
Africa, Cindy-Jade
Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
thesis_degree_str Doctoral
title Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
title_full Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
title_fullStr Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
title_full_unstemmed Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
title_short Investigation of microbial metal-sulfide interfacial environments under mineral bioleach simulated conditions
title_sort investigation of microbial metal sulfide interfacial environments under mineral bioleach simulated conditions
topic Bioprocess Engineering
url http://hdl.handle.net/11427/25001
work_keys_str_mv AT africacindyjade investigationofmicrobialmetalsulfideinterfacialenvironmentsundermineralbioleachsimulatedconditions