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The effect of cis-platinum alone or in combination with radiation on mouse lung
Cis-platinum is a widely used cytotoxic agent with known radiosensitising properties. It is used in the treatment of various types of lung cancer that may include radiation to the lung as part of the treatment protocol. There is little evidence and some conflict as to whether it sensitises pulmonary...
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| Format: | Thesis |
| Language: | English |
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Department of Medicine
2017
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| _version_ | 1867613956021944320 |
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| access_status_str | Open Access |
| author | Duffett, Rodger Vincent |
| author2 | Blekkenhorst, Gerhardus Hendrikus |
| author_browse | Blekkenhorst, Gerhardus Hendrikus Duffett, Rodger Vincent |
| author_facet | Blekkenhorst, Gerhardus Hendrikus Duffett, Rodger Vincent |
| author_sort | Duffett, Rodger Vincent |
| collection | Thesis |
| description | Cis-platinum is a widely used cytotoxic agent with known radiosensitising properties. It is used in the treatment of various types of lung cancer that may include radiation to the lung as part of the treatment protocol. There is little evidence and some conflict as to whether it sensitises pulmonary tissue to the effects of radiation treatment. This project investigates the effect of cis-platinum alone or in combination with radiation on mouse lung. Four end points were used to evaluate treatments. They were: the release of pulmonary surfactant, changes in breathing rate, a histology based score of damage and changes in TGF-β - a cytokine important in the development of fibrosis. Single doses of either cis-platinum or radiation, cis-platinum given immediately before a single dose of radiation, cis-platinum given immediately before the first of two fractions of radiation and cis-platinum given at various times before and after a single dose of radiation were investigated. Cis-platinum alone was observed to cause an increase in the phospholipid content of lavaged surfactant. Cis-platinum was observed to cause an early release in surfactant and a trend existed for it to induce an early increase in breathing rates as compared to that induced by radiation alone. Cis-platinum was observed to increase radiation damage as assessed using a histology based scoring system. Higher TGF-β levels in lavaged surfactant were observed in C57 /Bl mice as compared to Balb/C. No difference in TGF-β levels was seen in homogenised lung between the strains. Cis-platinum may cause changes in TGF-β in C57/Bl mice but further work is necessary to confirm this. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26352 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:44:22.820Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Department of Medicine |
| publisherStr | Department of Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26352 The effect of cis-platinum alone or in combination with radiation on mouse lung Duffett, Rodger Vincent Blekkenhorst, Gerhardus Hendrikus Abratt, Raymond Cisplatin - pharmacology Lung Neoplasms - drug therapy Cis-platinum is a widely used cytotoxic agent with known radiosensitising properties. It is used in the treatment of various types of lung cancer that may include radiation to the lung as part of the treatment protocol. There is little evidence and some conflict as to whether it sensitises pulmonary tissue to the effects of radiation treatment. This project investigates the effect of cis-platinum alone or in combination with radiation on mouse lung. Four end points were used to evaluate treatments. They were: the release of pulmonary surfactant, changes in breathing rate, a histology based score of damage and changes in TGF-β - a cytokine important in the development of fibrosis. Single doses of either cis-platinum or radiation, cis-platinum given immediately before a single dose of radiation, cis-platinum given immediately before the first of two fractions of radiation and cis-platinum given at various times before and after a single dose of radiation were investigated. Cis-platinum alone was observed to cause an increase in the phospholipid content of lavaged surfactant. Cis-platinum was observed to cause an early release in surfactant and a trend existed for it to induce an early increase in breathing rates as compared to that induced by radiation alone. Cis-platinum was observed to increase radiation damage as assessed using a histology based scoring system. Higher TGF-β levels in lavaged surfactant were observed in C57 /Bl mice as compared to Balb/C. No difference in TGF-β levels was seen in homogenised lung between the strains. Cis-platinum may cause changes in TGF-β in C57/Bl mice but further work is necessary to confirm this. 2017-11-17T07:47:10Z 2017-11-17T07:47:10Z 1999 2017-04-18T14:14:03Z Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/26352 eng application/pdf Department of Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Cisplatin - pharmacology Lung Neoplasms - drug therapy Duffett, Rodger Vincent The effect of cis-platinum alone or in combination with radiation on mouse lung |
| thesis_degree_str | Master's |
| title | The effect of cis-platinum alone or in combination with radiation on mouse lung |
| title_full | The effect of cis-platinum alone or in combination with radiation on mouse lung |
| title_fullStr | The effect of cis-platinum alone or in combination with radiation on mouse lung |
| title_full_unstemmed | The effect of cis-platinum alone or in combination with radiation on mouse lung |
| title_short | The effect of cis-platinum alone or in combination with radiation on mouse lung |
| title_sort | effect of cis platinum alone or in combination with radiation on mouse lung |
| topic | Cisplatin - pharmacology Lung Neoplasms - drug therapy |
| url | http://hdl.handle.net/11427/26352 |
| work_keys_str_mv | AT duffettrodgervincent theeffectofcisplatinumaloneorincombinationwithradiationonmouselung AT duffettrodgervincent effectofcisplatinumaloneorincombinationwithradiationonmouselung |