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Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes
Two separate assays for prizidilol have been developed. One relies on separation by high performance liquid chromatography, and the other on separation by thin layer chromatography. Both of these methods have several advantages over the existing assay developed by JC Pearce for Smith Kline and Frenc...
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| Format: | Thesis |
| Language: | English |
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Division of Clinical Pharmacology
2017
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| _version_ | 1867613140272807936 |
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| access_status_str | Open Access |
| author | Huang, Cheng-Yi |
| author2 | Folb, Peter I |
| author_browse | Folb, Peter I Huang, Cheng-Yi |
| author_facet | Folb, Peter I Huang, Cheng-Yi |
| author_sort | Huang, Cheng-Yi |
| collection | Thesis |
| description | Two separate assays for prizidilol have been developed. One relies on separation by high performance liquid chromatography, and the other on separation by thin layer chromatography. Both of these methods have several advantages over the existing assay developed by JC Pearce for Smith Kline and French Ltd. (SK & F). Firstly, both methods utilize pepsin to digest plasma protein since it is known that precipitation with trichloroacetic acid of the protein leads to losses by binding at different rates of prizidilol and of the internal standard. The SK & F assay might therefore be less sensitive and precise than necessary. Secondly, use of the complexing reagent, quinolin-3-al, leads to greater sensitivity of detection than the reagent, anisaldehyde, previously employed. The minimum level of quantitation of the developed assays are approximately 30 μg/1 by HPLC and 15 μg/1 by TLC, as against 50 μg/1 by the earlier HPLC method originating from SK & F laboratories. Finally, the TLC method is much quicker to perform than the previous assay. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26534 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:24.573Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Division of Clinical Pharmacology |
| publisherStr | Division of Clinical Pharmacology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26534 Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes Huang, Cheng-Yi Folb, Peter I Cridland, J S Clinical Pharmacology Two separate assays for prizidilol have been developed. One relies on separation by high performance liquid chromatography, and the other on separation by thin layer chromatography. Both of these methods have several advantages over the existing assay developed by JC Pearce for Smith Kline and French Ltd. (SK & F). Firstly, both methods utilize pepsin to digest plasma protein since it is known that precipitation with trichloroacetic acid of the protein leads to losses by binding at different rates of prizidilol and of the internal standard. The SK & F assay might therefore be less sensitive and precise than necessary. Secondly, use of the complexing reagent, quinolin-3-al, leads to greater sensitivity of detection than the reagent, anisaldehyde, previously employed. The minimum level of quantitation of the developed assays are approximately 30 μg/1 by HPLC and 15 μg/1 by TLC, as against 50 μg/1 by the earlier HPLC method originating from SK & F laboratories. Finally, the TLC method is much quicker to perform than the previous assay. 2017-12-11T10:20:37Z 2017-12-11T10:20:37Z 1984 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/26534 eng application/pdf Division of Clinical Pharmacology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Clinical Pharmacology Huang, Cheng-Yi Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes |
| thesis_degree_str | Doctoral |
| title | Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes |
| title_full | Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes |
| title_fullStr | Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes |
| title_full_unstemmed | Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes |
| title_short | Prizidilol : analytical methods and in vitro metabolism by hepatic enzymes |
| title_sort | prizidilol analytical methods and in vitro metabolism by hepatic enzymes |
| topic | Clinical Pharmacology |
| url | http://hdl.handle.net/11427/26534 |
| work_keys_str_mv | AT huangchengyi prizidilolanalyticalmethodsandinvitrometabolismbyhepaticenzymes |