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Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status
It has been reported that there is a 100% incidence of ulceration of the squamous pars oesophagea of the pig's stomach following bile duct ligation. The reproducibility of this model has made possible its use in the investigation of the biochemical aspects of mucus secretion in both the normal state...
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| Format: | Thesis |
| Language: | English |
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Department of Surgery
2017
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| _version_ | 1867613181694705664 |
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| access_status_str | Open Access |
| author | Mall, Anwarul Haq |
| author2 | Hickman, Rosemary |
| author_browse | Hickman, Rosemary Mall, Anwarul Haq |
| author_facet | Hickman, Rosemary Mall, Anwarul Haq |
| author_sort | Mall, Anwarul Haq |
| collection | Thesis |
| description | It has been reported that there is a 100% incidence of ulceration of the squamous pars oesophagea of the pig's stomach following bile duct ligation. The reproducibility of this model has made possible its use in the investigation of the biochemical aspects of mucus secretion in both the normal state and at various states of ulceration. The main findings are summarised below: Mucus scrapings of the cardiac gland region of the pig stomach had a higher water and total protein content in the pre-ulcerated, ulcerated and post-ulcerated states. Gel chromatography on Sepharose CL-4B indicated larger amounts of degraded mucins relative to native mucins in the samples obtained from pre-ulcerated, ulcerated and post-ulcerated stomachs, as compared with the normal and control samples. The amounts of purified mucins obtained after isopycnic centrifugation in CsCl and gel chromatography on Sepharose CL-2B decreased from the normals and controls to the bile duct-ligated pigs. An analysis on SDS-PAGE revealed a considerable degree of proteolytic degradation of the pig gastric mucins in the bile duct-ligated pigs as compared with the normal and control animals. Staining reagents specific for both the protein and the carbohydrate components of the mucus glycoprotein were employed in gel electrophoresis, which also revealed the presence of contaminating protein, viz. haemoglobin, pepsin, albumin and smaller glycoproteins, to a greater extent in the ulcerated than in the normal and control states. Since pig gastric mucins contain 75% of carbohydrate, the determination of the proportions of monosaccharide constituents was essential. This was performed by GLC analysis of the alditol acetate derivatives of the sugars, which were characterised by mass spectrometry as well as by their retention times, relative to standards, on both packed and capillary columns. The most striking changes indicated by the GLC analyses were a decrease in the fucose content of the mucins from the normal to the ulcerated states and an increase in the proportion of N-acetylglucosamine in mucins from sham-operated animals. The control (sham-operated) pig behaved very similarly to the normal pig for up to 24 hours after the surgical procedure. At 48 hours, however, slight changes resembling those following bile duct ligation occurred. This could be due to the trauma of the sham-operation, which involved the surgical insertion of a cannula in the body region of the pig's stomach. It is possible that, under such stress biosynthesis of the mucins may be affected. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26538 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:03.909Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Department of Surgery |
| publisherStr | Department of Surgery |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26538 Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status Mall, Anwarul Haq Hickman, Rosemary Surgery It has been reported that there is a 100% incidence of ulceration of the squamous pars oesophagea of the pig's stomach following bile duct ligation. The reproducibility of this model has made possible its use in the investigation of the biochemical aspects of mucus secretion in both the normal state and at various states of ulceration. The main findings are summarised below: Mucus scrapings of the cardiac gland region of the pig stomach had a higher water and total protein content in the pre-ulcerated, ulcerated and post-ulcerated states. Gel chromatography on Sepharose CL-4B indicated larger amounts of degraded mucins relative to native mucins in the samples obtained from pre-ulcerated, ulcerated and post-ulcerated stomachs, as compared with the normal and control samples. The amounts of purified mucins obtained after isopycnic centrifugation in CsCl and gel chromatography on Sepharose CL-2B decreased from the normals and controls to the bile duct-ligated pigs. An analysis on SDS-PAGE revealed a considerable degree of proteolytic degradation of the pig gastric mucins in the bile duct-ligated pigs as compared with the normal and control animals. Staining reagents specific for both the protein and the carbohydrate components of the mucus glycoprotein were employed in gel electrophoresis, which also revealed the presence of contaminating protein, viz. haemoglobin, pepsin, albumin and smaller glycoproteins, to a greater extent in the ulcerated than in the normal and control states. Since pig gastric mucins contain 75% of carbohydrate, the determination of the proportions of monosaccharide constituents was essential. This was performed by GLC analysis of the alditol acetate derivatives of the sugars, which were characterised by mass spectrometry as well as by their retention times, relative to standards, on both packed and capillary columns. The most striking changes indicated by the GLC analyses were a decrease in the fucose content of the mucins from the normal to the ulcerated states and an increase in the proportion of N-acetylglucosamine in mucins from sham-operated animals. The control (sham-operated) pig behaved very similarly to the normal pig for up to 24 hours after the surgical procedure. At 48 hours, however, slight changes resembling those following bile duct ligation occurred. This could be due to the trauma of the sham-operation, which involved the surgical insertion of a cannula in the body region of the pig's stomach. It is possible that, under such stress biosynthesis of the mucins may be affected. 2017-12-11T10:21:30Z 2017-12-11T10:21:30Z 1984 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/26538 eng application/pdf Department of Surgery Faculty of Health Sciences University of Cape Town |
| spellingShingle | Surgery Mall, Anwarul Haq Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| thesis_degree_str | Master's |
| title | Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| title_full | Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| title_fullStr | Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| title_full_unstemmed | Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| title_short | Physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| title_sort | physical and chemical changes in porcine gastric mucus in the normal and ulcerated status |
| topic | Surgery |
| url | http://hdl.handle.net/11427/26538 |
| work_keys_str_mv | AT mallanwarulhaq physicalandchemicalchangesinporcinegastricmucusinthenormalandulceratedstatus |