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The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli
Fluoroquinolone susceptible and resistant Campylobacter coli were isolated from pigs on two separate pig farms. C. coli are enteric pathogens of humans and animals and although diarrhoea resulting from C. coli and C. jejuni is generally a self-limiting disease, in severe cases, fluoroquinolones are...
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| Format: | Thesis |
| Language: | English |
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Division of Medical Microbiology
2017
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| _version_ | 1867613234888966144 |
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| access_status_str | Open Access |
| author | Cooper, Rhett |
| author2 | Elisha, B Gay |
| author_browse | Cooper, Rhett Elisha, B Gay |
| author_facet | Elisha, B Gay Cooper, Rhett |
| author_sort | Cooper, Rhett |
| collection | Thesis |
| description | Fluoroquinolone susceptible and resistant Campylobacter coli were isolated from pigs on two separate pig farms. C. coli are enteric pathogens of humans and animals and although diarrhoea resulting from C. coli and C. jejuni is generally a self-limiting disease, in severe cases, fluoroquinolones are the choice antibiotic for treatment. The presence of fluoroquinolone resistant C. coli strains in the food chain is cause for concern as this may be a source of resistant strains in humans. Sixty-one isolates were included in the study: 26 were susceptible to nalidixic acid and ciprofloxacin and 35 were resistant to these antibiotics. Fifty-five strains were obtained from pigs on farm A, while 6 strains were obtained from pigs on farm B, the source farm of pigs to farm A. Serotyping and flaA typing were carried out to study the epidemiology of the isolates. Serotyping identified 0:24 (11/61) as the most frequent serotype isolated, followed by 0:5 (7/61). Common serotypes 0:48, 0:54 and 0:59 were identified in strains from both farms. A high number of the strains were non-typeable (23/61) but were distinguished by flaA typing. RFLP analysis of the flaA gene revealed 13 distinct profiles in strains from farm A, and 4 profiles in strains from farm B, of which only 1 was unique to farm B. Profile 1 was the commonest profile observed with 31 % (17 /55) of flaA typed strains in this profile. There was an association between 0:24, profile 6, and resistance. Resistant and sensitive pairs were isolated from 15 pigs; flaA profiles of each of 4 pairs were identical, suggesting selection of resistant mutants from previously sensitive populations. An investigation of the molecular basis of the fluoroquinolone resistance identified a Thr-86 to Ile mutation in GyrA, the primary target of these antibiotics. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26542 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:54.720Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Division of Medical Microbiology |
| publisherStr | Division of Medical Microbiology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26542 The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli Cooper, Rhett Elisha, B Gay Lastovica, Albert Medical Microbiology Fluoroquinolone susceptible and resistant Campylobacter coli were isolated from pigs on two separate pig farms. C. coli are enteric pathogens of humans and animals and although diarrhoea resulting from C. coli and C. jejuni is generally a self-limiting disease, in severe cases, fluoroquinolones are the choice antibiotic for treatment. The presence of fluoroquinolone resistant C. coli strains in the food chain is cause for concern as this may be a source of resistant strains in humans. Sixty-one isolates were included in the study: 26 were susceptible to nalidixic acid and ciprofloxacin and 35 were resistant to these antibiotics. Fifty-five strains were obtained from pigs on farm A, while 6 strains were obtained from pigs on farm B, the source farm of pigs to farm A. Serotyping and flaA typing were carried out to study the epidemiology of the isolates. Serotyping identified 0:24 (11/61) as the most frequent serotype isolated, followed by 0:5 (7/61). Common serotypes 0:48, 0:54 and 0:59 were identified in strains from both farms. A high number of the strains were non-typeable (23/61) but were distinguished by flaA typing. RFLP analysis of the flaA gene revealed 13 distinct profiles in strains from farm A, and 4 profiles in strains from farm B, of which only 1 was unique to farm B. Profile 1 was the commonest profile observed with 31 % (17 /55) of flaA typed strains in this profile. There was an association between 0:24, profile 6, and resistance. Resistant and sensitive pairs were isolated from 15 pigs; flaA profiles of each of 4 pairs were identical, suggesting selection of resistant mutants from previously sensitive populations. An investigation of the molecular basis of the fluoroquinolone resistance identified a Thr-86 to Ile mutation in GyrA, the primary target of these antibiotics. 2017-12-11T14:13:56Z 2017-12-11T14:13:56Z 2001 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/26542 eng application/pdf Division of Medical Microbiology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medical Microbiology Cooper, Rhett The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli |
| thesis_degree_str | Master's |
| title | The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli |
| title_full | The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli |
| title_fullStr | The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli |
| title_full_unstemmed | The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli |
| title_short | The epidemiology & molecular basis of fluoroquinolone resistant & susceptible isolates of Campylobacter coli |
| title_sort | epidemiology molecular basis of fluoroquinolone resistant susceptible isolates of campylobacter coli |
| topic | Medical Microbiology |
| url | http://hdl.handle.net/11427/26542 |
| work_keys_str_mv | AT cooperrhett theepidemiologymolecularbasisoffluoroquinoloneresistantsusceptibleisolatesofcampylobactercoli AT cooperrhett epidemiologymolecularbasisoffluoroquinoloneresistantsusceptibleisolatesofcampylobactercoli |