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Post-translational processing of the low density lipoprotein receptor
The low density lipoprotein (LDL) receptor is a transmembrane glycoprotein that mediates the uptake of plasma LDL and thereby provides cholesterol to cells. During its synthesis in the endoplasmic reticulum, the LDL receptor folds and forms disulfide bonds in multiple cysteine-rich repeats. N- and 0...
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| Format: | Thesis |
| Language: | English |
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Division of Medical Biochemistry and Structural Biology
2017
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| _version_ | 1867613330887147520 |
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| access_status_str | Open Access |
| author | Ozinsky, Adrian |
| author2 | Van der Westhuyzen, Deneys R |
| author_browse | Ozinsky, Adrian Van der Westhuyzen, Deneys R |
| author_facet | Van der Westhuyzen, Deneys R Ozinsky, Adrian |
| author_sort | Ozinsky, Adrian |
| collection | Thesis |
| description | The low density lipoprotein (LDL) receptor is a transmembrane glycoprotein that mediates the uptake of plasma LDL and thereby provides cholesterol to cells. During its synthesis in the endoplasmic reticulum, the LDL receptor folds and forms disulfide bonds in multiple cysteine-rich repeats. N- and 0-linked oligosaccharide chains are added in the endoplasmic reticulum and processed during passage through the Golgi apparatus, en route to the cell surface. The aim of this thesis was to study the influence of post-translational events on the synthesis of the LDL receptor. Experiments addressed: 1) the necessity of the compartmental organisation of the secretory pathway for the glycosylation of the LDL receptor; 2) the requirements for the formation of disulfide bonds; 3) the role for the chaperone, calnexin, in the folding of the LDL receptor; and 4) the manner in which folding was disrupted by mutations. Experiments were performed in cultured cells that were incubated with [³⁵S]methionine. Biosynthetically-labelled LDL receptor was immunoprecipitated and was analysed by SOS polyacrylamide gel electrophoresis. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26672 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:25.395Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Division of Medical Biochemistry and Structural Biology |
| publisherStr | Division of Medical Biochemistry and Structural Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26672 Post-translational processing of the low density lipoprotein receptor Ozinsky, Adrian Van der Westhuyzen, Deneys R Receptors, LDL - biosynthesis Receptors, LDL - chemistry Medical Biochemistry The low density lipoprotein (LDL) receptor is a transmembrane glycoprotein that mediates the uptake of plasma LDL and thereby provides cholesterol to cells. During its synthesis in the endoplasmic reticulum, the LDL receptor folds and forms disulfide bonds in multiple cysteine-rich repeats. N- and 0-linked oligosaccharide chains are added in the endoplasmic reticulum and processed during passage through the Golgi apparatus, en route to the cell surface. The aim of this thesis was to study the influence of post-translational events on the synthesis of the LDL receptor. Experiments addressed: 1) the necessity of the compartmental organisation of the secretory pathway for the glycosylation of the LDL receptor; 2) the requirements for the formation of disulfide bonds; 3) the role for the chaperone, calnexin, in the folding of the LDL receptor; and 4) the manner in which folding was disrupted by mutations. Experiments were performed in cultured cells that were incubated with [³⁵S]methionine. Biosynthetically-labelled LDL receptor was immunoprecipitated and was analysed by SOS polyacrylamide gel electrophoresis. 2017-12-14T14:20:10Z 2017-12-14T14:20:10Z 1996 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/26672 eng application/pdf Division of Medical Biochemistry and Structural Biology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Receptors, LDL - biosynthesis Receptors, LDL - chemistry Medical Biochemistry Ozinsky, Adrian Post-translational processing of the low density lipoprotein receptor |
| thesis_degree_str | Doctoral |
| title | Post-translational processing of the low density lipoprotein receptor |
| title_full | Post-translational processing of the low density lipoprotein receptor |
| title_fullStr | Post-translational processing of the low density lipoprotein receptor |
| title_full_unstemmed | Post-translational processing of the low density lipoprotein receptor |
| title_short | Post-translational processing of the low density lipoprotein receptor |
| title_sort | post translational processing of the low density lipoprotein receptor |
| topic | Receptors, LDL - biosynthesis Receptors, LDL - chemistry Medical Biochemistry |
| url | http://hdl.handle.net/11427/26672 |
| work_keys_str_mv | AT ozinskyadrian posttranslationalprocessingofthelowdensitylipoproteinreceptor |