Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos)
I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seve...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Biological Sciences
2017
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613343347376128 |
|---|---|
| access_status_str | Open Access |
| author | Potts, Alistair |
| author2 | Verboom, George Anthony |
| author_browse | Potts, Alistair Verboom, George Anthony |
| author_facet | Verboom, George Anthony Potts, Alistair |
| author_sort | Potts, Alistair |
| collection | Thesis |
| description | I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seven morphological variables grouped the samples into three different growth forms, specifically an erect-form, a prostrate-form and a shrub-form. The genetic variability of four plastid markers and one nuclear marker was determined for seven individuals selected from the populations. The trnL'UAAF-trnFGAA plastid marker was used to amplify the remaining samples and revealed four haplotypes, with a basal haplotype fixed in both the prostrate form and a single population of the erect form, a unique haplotype fixed in the shrub form, and a mix of two other haplotypes in the remaining erect forms. The congruence between haplotypes and regeneration strategy suggests a longstanding pattern of restricted gene flow. However, a small sample size associated with the plastid marker data limits or reduces the certainty of these findings. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26701 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:38.153Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2017 |
| publishDateRange | 2017 |
| publishDateSort | 2017 |
| publisher | Department of Biological Sciences |
| publisherStr | Department of Biological Sciences |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26701 Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) Potts, Alistair Verboom, George Anthony Hoffmann, Timm Botany Plant Ecology I investigated the infraspecific diversity among individuals of Aspalathus linearis from five localities in the Cederberg Fynbos region using morphological traits and two DNA- based methods: inter-simple sequence repeats (ISSR) and a plastid marker. A principle component analysis (PCA) based on seven morphological variables grouped the samples into three different growth forms, specifically an erect-form, a prostrate-form and a shrub-form. The genetic variability of four plastid markers and one nuclear marker was determined for seven individuals selected from the populations. The trnL'UAAF-trnFGAA plastid marker was used to amplify the remaining samples and revealed four haplotypes, with a basal haplotype fixed in both the prostrate form and a single population of the erect form, a unique haplotype fixed in the shrub form, and a mix of two other haplotypes in the remaining erect forms. The congruence between haplotypes and regeneration strategy suggests a longstanding pattern of restricted gene flow. However, a small sample size associated with the plastid marker data limits or reduces the certainty of these findings. 2017-12-15T07:06:49Z 2017-12-15T07:06:49Z 2006 2017-02-10T10:09:47Z Bachelor Thesis Honours BSc (Hons) http://hdl.handle.net/11427/26701 eng application/pdf Department of Biological Sciences Faculty of Science University of Cape Town |
| spellingShingle | Botany Plant Ecology Potts, Alistair Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) |
| thesis_degree_str | Bachelor's / Honours |
| title | Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) |
| title_full | Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) |
| title_fullStr | Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) |
| title_full_unstemmed | Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) |
| title_short | Morphological and molecular analyses for the characterization of Aspalanthus linearis (Fabaceae: rooibos) |
| title_sort | morphological and molecular analyses for the characterization of aspalanthus linearis fabaceae rooibos |
| topic | Botany Plant Ecology |
| url | http://hdl.handle.net/11427/26701 |
| work_keys_str_mv | AT pottsalistair morphologicalandmolecularanalysesforthecharacterizationofaspalanthuslinearisfabaceaerooibos |