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Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT)
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Chemical Pathology
2014
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| _version_ | 1867613341953818624 |
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| access_status_str | Open Access |
| author | Mbewe, Boniface |
| author2 | Mcintosh, David B |
| author_browse | Mbewe, Boniface Mcintosh, David B |
| author_facet | Mcintosh, David B Mbewe, Boniface |
| author_sort | Mbewe, Boniface |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/2696 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:36.552Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Chemical Pathology |
| publisherStr | Division of Chemical Pathology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/2696 Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) Mbewe, Boniface Mcintosh, David B Chibale, Kelly Chemical Pathology Includes bibliographical references. The research concerns sub-cloning the gene for HGXPRT from Plasmodium falciparum from a vector with a His-tag facility to one without, expression of the protein in E. coli, and purification. On an analytical scale (40 ml culture), a purification procedure was developed that involves extraction of contaminating proteins by anion exchange chromatography (HGXPRT does not bind under the conditions used), followed by Reactive Red 120 agarose affinity chromatography. 2014-07-28T08:14:03Z 2014-07-28T08:14:03Z 2005 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/2696 eng application/pdf Division of Chemical Pathology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Chemical Pathology Mbewe, Boniface Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) |
| thesis_degree_str | Doctoral |
| title | Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) |
| title_full | Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) |
| title_fullStr | Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) |
| title_full_unstemmed | Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) |
| title_short | Cloning, expression, purification and drug targeting of Plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase (HGXPRT) |
| title_sort | cloning expression purification and drug targeting of plasmodium falciparum hypoxanthine guanine xanthine phosphoribosyltransferase hgxprt |
| topic | Chemical Pathology |
| url | http://hdl.handle.net/11427/2696 |
| work_keys_str_mv | AT mbeweboniface cloningexpressionpurificationanddrugtargetingofplasmodiumfalciparumhypoxanthineguaninexanthinephosphoribosyltransferasehgxprt |