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Induction of the retinal pigment epithelium of the chicken embryonic eye
During development of the eye, invagination of the optic cup gives rise to a double layered neuroepithelium, part of which differentiates into the retinal pigment epithelium (RPE). The molecular mechanisms which control differentiation of the RPE are not known. The present study was undertaken to de...
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| Format: | Thesis |
| Language: | English |
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Division of Cell Biology
2018
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| _version_ | 1867613299632242688 |
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| access_status_str | Open Access |
| author | Franz, Tamara Anne |
| author2 | Kidson, Sue |
| author_browse | Franz, Tamara Anne Kidson, Sue |
| author_facet | Kidson, Sue Franz, Tamara Anne |
| author_sort | Franz, Tamara Anne |
| collection | Thesis |
| description | During development of the eye, invagination of the optic cup gives rise to a double layered neuroepithelium, part of which differentiates into the retinal pigment epithelium (RPE). The molecular mechanisms which control differentiation of the RPE are not known. The present study was undertaken to determine 1) when induction of the RPE has occurred in chicken embryos and 2) to investigate whether contact with the presumptive neural retina (NR) is required for RPE differentiation. In order to investigate when RPE induction has occurred, early expression of two genes involved in pigmentation were investigated. Digoxigenin-labeled tyrosinase and tyrosinaserelated protein-2 (TRP-2) riboprobes were synthesised and used in ISH reactions on embryonic eye tissue. Tyrosinase transcripts were first detected at stage 19.5 (70-71 hours) and TRP-2 transcripts were detected a few hours earlier at stage 18.5 (67-69 hours) of embryonic development. These results indicate that induction has occurred by stage 18.5, approximately ten hours before distinct granules are visible in the RPE. The tyrosinase and TRP-2 transcripts were always localised first in the optical axis of the eye in the region where pigment granules are first present. This indicates that differentiation of the RPE proceeds from the optical axis of the eye cup outwards towards the periphery and that induction of the RPE may also proceed in this direction. To determine whether the presumptive NR is required for RPE induction, synthetic barriers were inserted into the uninvaginated optic vesicle of chicken embryos at stage 11 (40-45 hours) of development. The embryos were cultured in vitro until the optic vesicle had invaginated and sectioned to locate the barrier. Results suggest that contact with the presumptive NR may not be necessary for RPE induction. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/26993 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:55.830Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Cell Biology |
| publisherStr | Division of Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/26993 Induction of the retinal pigment epithelium of the chicken embryonic eye Franz, Tamara Anne Kidson, Sue Cell Biology During development of the eye, invagination of the optic cup gives rise to a double layered neuroepithelium, part of which differentiates into the retinal pigment epithelium (RPE). The molecular mechanisms which control differentiation of the RPE are not known. The present study was undertaken to determine 1) when induction of the RPE has occurred in chicken embryos and 2) to investigate whether contact with the presumptive neural retina (NR) is required for RPE differentiation. In order to investigate when RPE induction has occurred, early expression of two genes involved in pigmentation were investigated. Digoxigenin-labeled tyrosinase and tyrosinaserelated protein-2 (TRP-2) riboprobes were synthesised and used in ISH reactions on embryonic eye tissue. Tyrosinase transcripts were first detected at stage 19.5 (70-71 hours) and TRP-2 transcripts were detected a few hours earlier at stage 18.5 (67-69 hours) of embryonic development. These results indicate that induction has occurred by stage 18.5, approximately ten hours before distinct granules are visible in the RPE. The tyrosinase and TRP-2 transcripts were always localised first in the optical axis of the eye in the region where pigment granules are first present. This indicates that differentiation of the RPE proceeds from the optical axis of the eye cup outwards towards the periphery and that induction of the RPE may also proceed in this direction. To determine whether the presumptive NR is required for RPE induction, synthetic barriers were inserted into the uninvaginated optic vesicle of chicken embryos at stage 11 (40-45 hours) of development. The embryos were cultured in vitro until the optic vesicle had invaginated and sectioned to locate the barrier. Results suggest that contact with the presumptive NR may not be necessary for RPE induction. 2018-01-25T13:59:17Z 2018-01-25T13:59:17Z 1997 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/26993 eng application/pdf Division of Cell Biology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Cell Biology Franz, Tamara Anne Induction of the retinal pigment epithelium of the chicken embryonic eye |
| thesis_degree_str | Master's |
| title | Induction of the retinal pigment epithelium of the chicken embryonic eye |
| title_full | Induction of the retinal pigment epithelium of the chicken embryonic eye |
| title_fullStr | Induction of the retinal pigment epithelium of the chicken embryonic eye |
| title_full_unstemmed | Induction of the retinal pigment epithelium of the chicken embryonic eye |
| title_short | Induction of the retinal pigment epithelium of the chicken embryonic eye |
| title_sort | induction of the retinal pigment epithelium of the chicken embryonic eye |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/26993 |
| work_keys_str_mv | AT franztamaraanne inductionoftheretinalpigmentepitheliumofthechickenembryoniceye |