Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes
The present study was undertaken to establish the mechanism(s) involved in the endocytic pathway, in particular, early endosome processing and delivery to the lysosomes. Two models for endosome processing have previously been proposed in the literature, namely the maturation and vesicular traffic mo...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Medical Biochemistry and Structural Biology
2018
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613521192157184 |
|---|---|
| access_status_str | Open Access |
| author | Stroud, Evelyn Joy |
| author2 | Thilo, Lutz |
| author_browse | Stroud, Evelyn Joy Thilo, Lutz |
| author_facet | Thilo, Lutz Stroud, Evelyn Joy |
| author_sort | Stroud, Evelyn Joy |
| collection | Thesis |
| description | The present study was undertaken to establish the mechanism(s) involved in the endocytic pathway, in particular, early endosome processing and delivery to the lysosomes. Two models for endosome processing have previously been proposed in the literature, namely the maturation and vesicular traffic models. The general consensus has been an early phase of intermingling of the endocytic contents markers within early endosomes that mature to form non-fusogenic late endosomes (maturation model). This maturation phase is followed by a segregation phase where intermingling of contents between vesicles no longer takes place. To establish the mechanism(s) involved in early endosome processing and delivery to lysosomes, a kinetic analysis was made using results from cellular fluid-phase uptake assays. This unique approach offers an alternative view to previous studies on the mechanisms in operation during endocytic processing. The results and conclusions made could thus confirm or disprove previously proposed mechanisms. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27043 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:37:28.134Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Medical Biochemistry and Structural Biology |
| publisherStr | Division of Medical Biochemistry and Structural Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27043 Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes Stroud, Evelyn Joy Thilo, Lutz Medical Biochemistry Biological transport Endosomes Lysosomes The present study was undertaken to establish the mechanism(s) involved in the endocytic pathway, in particular, early endosome processing and delivery to the lysosomes. Two models for endosome processing have previously been proposed in the literature, namely the maturation and vesicular traffic models. The general consensus has been an early phase of intermingling of the endocytic contents markers within early endosomes that mature to form non-fusogenic late endosomes (maturation model). This maturation phase is followed by a segregation phase where intermingling of contents between vesicles no longer takes place. To establish the mechanism(s) involved in early endosome processing and delivery to lysosomes, a kinetic analysis was made using results from cellular fluid-phase uptake assays. This unique approach offers an alternative view to previous studies on the mechanisms in operation during endocytic processing. The results and conclusions made could thus confirm or disprove previously proposed mechanisms. 2018-01-29T07:08:23Z 2018-01-29T07:08:23Z 1995 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/27043 eng application/pdf Division of Medical Biochemistry and Structural Biology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medical Biochemistry Biological transport Endosomes Lysosomes Stroud, Evelyn Joy Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes |
| thesis_degree_str | Master's |
| title | Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes |
| title_full | Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes |
| title_fullStr | Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes |
| title_full_unstemmed | Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes |
| title_short | Kinetic analysis of endosome processing : maturation of early endosomes and vesicular traffic to lysosomes |
| title_sort | kinetic analysis of endosome processing maturation of early endosomes and vesicular traffic to lysosomes |
| topic | Medical Biochemistry Biological transport Endosomes Lysosomes |
| url | http://hdl.handle.net/11427/27043 |
| work_keys_str_mv | AT stroudevelynjoy kineticanalysisofendosomeprocessingmaturationofearlyendosomesandvesiculartraffictolysosomes |