Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Characterisation of the human α2(I) procollagen promoter-binding proteins
In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study ha...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Medical Biochemistry and Structural Biology
2018
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613268592295936 |
|---|---|
| access_status_str | Open Access |
| author | Collins, Malcolm Robert |
| author2 | Parker, M Iqbal |
| author_browse | Collins, Malcolm Robert Parker, M Iqbal |
| author_facet | Parker, M Iqbal Collins, Malcolm Robert |
| author_sort | Collins, Malcolm Robert |
| collection | Thesis |
| description | In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study had previously been transformed with either simian virus 40 (SVWI-38) or by γ-radiation (CT-1). The SVWI-38 fibroblasts do not produce any α2(I) collagen chains, whereas the CT-1 cell line produces normal type I collagen. Previous studies suggested that trans-acting factor(s) may be responsible for the inactivation of the α2(I) procollagen gene in SVWI-38 fibroblasts (Parker et. al. (1989) J. Biol. Chem 264, 7147-7152; Parker et. al. (1992) Nucleic Acids Res. 20, 5825-5830). In this study, the SVWI-38 proximal promoter (-350 to +54) was sequenced and shown to be normal, thereby ruling out any possibility that mutations within this region was responsible for inactivation of the gene. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27139 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:26.520Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Medical Biochemistry and Structural Biology |
| publisherStr | Division of Medical Biochemistry and Structural Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27139 Characterisation of the human α2(I) procollagen promoter-binding proteins Collins, Malcolm Robert Parker, M Iqbal DNA-Binding Proteins - analysis Procollagen - analysis Protein binding In an attempt to elucidate the transcriptional mechanisms that regulate the expression of the human α2(I) procollagen gene, cis-acting DNA-elements within the proximal promoter were identified and their corresponding trans-acting factors characterised. The fibroblast cell lines used in this study had previously been transformed with either simian virus 40 (SVWI-38) or by γ-radiation (CT-1). The SVWI-38 fibroblasts do not produce any α2(I) collagen chains, whereas the CT-1 cell line produces normal type I collagen. Previous studies suggested that trans-acting factor(s) may be responsible for the inactivation of the α2(I) procollagen gene in SVWI-38 fibroblasts (Parker et. al. (1989) J. Biol. Chem 264, 7147-7152; Parker et. al. (1992) Nucleic Acids Res. 20, 5825-5830). In this study, the SVWI-38 proximal promoter (-350 to +54) was sequenced and shown to be normal, thereby ruling out any possibility that mutations within this region was responsible for inactivation of the gene. 2018-01-30T14:00:25Z 2018-01-30T14:00:25Z 1993 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/27139 eng application/pdf Division of Medical Biochemistry and Structural Biology Faculty of Health Sciences University of Cape Town |
| spellingShingle | DNA-Binding Proteins - analysis Procollagen - analysis Protein binding Collins, Malcolm Robert Characterisation of the human α2(I) procollagen promoter-binding proteins |
| thesis_degree_str | Doctoral |
| title | Characterisation of the human α2(I) procollagen promoter-binding proteins |
| title_full | Characterisation of the human α2(I) procollagen promoter-binding proteins |
| title_fullStr | Characterisation of the human α2(I) procollagen promoter-binding proteins |
| title_full_unstemmed | Characterisation of the human α2(I) procollagen promoter-binding proteins |
| title_short | Characterisation of the human α2(I) procollagen promoter-binding proteins |
| title_sort | characterisation of the human α2 i procollagen promoter binding proteins |
| topic | DNA-Binding Proteins - analysis Procollagen - analysis Protein binding |
| url | http://hdl.handle.net/11427/27139 |
| work_keys_str_mv | AT collinsmalcolmrobert characterisationofthehumana2iprocollagenpromoterbindingproteins |