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Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis
Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymaticall...
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| Format: | Thesis |
| Language: | English |
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Division of Medical Biochemistry and Structural Biology
2018
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| _version_ | 1867613337895829504 |
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| access_status_str | Open Access |
| author | Godenir, Nicole |
| author2 | Thilo, Lutz |
| author_browse | Godenir, Nicole Thilo, Lutz |
| author_facet | Thilo, Lutz Godenir, Nicole |
| author_sort | Godenir, Nicole |
| collection | Thesis |
| description | Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymatically with ³H- and ¹⁴C-galactose, respectively, to provide covalent membrane markers. Experiments were done to quantitate membrane traffic during endocytosis at the respective plasma membrane domains and that due to transcytosis. Internalized label was quantitatively distinguished from label on the respective cell surface by its resistance to removal by glycosidases. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27141 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:32.198Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Medical Biochemistry and Structural Biology |
| publisherStr | Division of Medical Biochemistry and Structural Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27141 Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis Godenir, Nicole Thilo, Lutz Medical Biochemistry Biological transport Cell membrane permeability. Endocytosis Kidney - citology Membranes - Physiology Membrane traffic in polarised cells was investigated by growing Madin-Darby canine kidney (MOCK) cells on ·permeable polycarbonate filter supports which allowed access to both sides of the cell monolayer. Membrane glycoconjugates on the apical and basolateral cell surfaces were labelled enzymatically with ³H- and ¹⁴C-galactose, respectively, to provide covalent membrane markers. Experiments were done to quantitate membrane traffic during endocytosis at the respective plasma membrane domains and that due to transcytosis. Internalized label was quantitatively distinguished from label on the respective cell surface by its resistance to removal by glycosidases. 2018-01-30T14:00:31Z 2018-01-30T14:00:31Z 1991 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/27141 eng Division of Medical Biochemistry and Structural Biology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medical Biochemistry Biological transport Cell membrane permeability. Endocytosis Kidney - citology Membranes - Physiology Godenir, Nicole Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| thesis_degree_str | Master's |
| title | Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| title_full | Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| title_fullStr | Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| title_full_unstemmed | Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| title_short | Membrane shedding in kidney (MDCK) cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| title_sort | membrane shedding in kidney mdck cells as revealed by covalent markers during quantification of endocytosis and transcytosis |
| topic | Medical Biochemistry Biological transport Cell membrane permeability. Endocytosis Kidney - citology Membranes - Physiology |
| url | http://hdl.handle.net/11427/27141 |
| work_keys_str_mv | AT godenirnicole membranesheddinginkidneymdckcellsasrevealedbycovalentmarkersduringquantificationofendocytosisandtranscytosis |