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An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram
Although there have been two-dimensional electrophoretic studies on fetal brain tissue (for instance, Yoshida and Takahashi, 1980), the emphasis in most of this work has been on developmental changes in protein expression, and not on the effects that drugs have on fetal brain protein complement. Klo...
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| Format: | Thesis |
| Language: | English |
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Division of Clinical Pharmacology
2018
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| _version_ | 1867613142798827520 |
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| access_status_str | Open Access |
| author | Heiberg, Ludvig |
| author2 | Folb, Peter I |
| author_browse | Folb, Peter I Heiberg, Ludvig |
| author_facet | Folb, Peter I Heiberg, Ludvig |
| author_sort | Heiberg, Ludvig |
| collection | Thesis |
| description | Although there have been two-dimensional electrophoretic studies on fetal brain tissue (for instance, Yoshida and Takahashi, 1980), the emphasis in most of this work has been on developmental changes in protein expression, and not on the effects that drugs have on fetal brain protein complement. Klose and co-workers (1977) did an early study using two-dimensional gel electrophoresis to determine the effects of various teratogens on whole embryos. No protein changes were found and that line of research was not continued. In this study two-dimensional gel electrophoresis is extensively used, in the belief that the usefulness of this technique to experimental teratology has not been fully evaluated. It is reasonable to suppose that a central nervous system teratogen administered during critical periods of susceptibility will led to perturbations of orderly brain development, and that these perturbations will be reflected as changes to the protein complement. The total brain protein complement of mice that have been exposed to drugs in utero will therefore be analysed, in the hope that any inductions or deletions of proteins as a result of drug exposure may provide a clue to the molecular events underlying drug injury to the fetus. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27187 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:26.417Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Clinical Pharmacology |
| publisherStr | Division of Clinical Pharmacology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27187 An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram Heiberg, Ludvig Folb, Peter I Disulfiram - Toxicology Phenytoin - Toxicology Fetus - Effect of drugs on Teratogenic agents Disulfiram - Toxicology Phenytoin - toxicity Fetal proteins - drug effects Fetus - Drug effects Tera Although there have been two-dimensional electrophoretic studies on fetal brain tissue (for instance, Yoshida and Takahashi, 1980), the emphasis in most of this work has been on developmental changes in protein expression, and not on the effects that drugs have on fetal brain protein complement. Klose and co-workers (1977) did an early study using two-dimensional gel electrophoresis to determine the effects of various teratogens on whole embryos. No protein changes were found and that line of research was not continued. In this study two-dimensional gel electrophoresis is extensively used, in the belief that the usefulness of this technique to experimental teratology has not been fully evaluated. It is reasonable to suppose that a central nervous system teratogen administered during critical periods of susceptibility will led to perturbations of orderly brain development, and that these perturbations will be reflected as changes to the protein complement. The total brain protein complement of mice that have been exposed to drugs in utero will therefore be analysed, in the hope that any inductions or deletions of proteins as a result of drug exposure may provide a clue to the molecular events underlying drug injury to the fetus. 2018-01-31T13:51:15Z 2018-01-31T13:51:15Z 1990 Master Thesis Masters MSc (Med) http://hdl.handle.net/11427/27187 eng application/pdf Division of Clinical Pharmacology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Disulfiram - Toxicology Phenytoin - Toxicology Fetus - Effect of drugs on Teratogenic agents Disulfiram - Toxicology Phenytoin - toxicity Fetal proteins - drug effects Fetus - Drug effects Tera Heiberg, Ludvig An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| thesis_degree_str | Master's |
| title | An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| title_full | An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| title_fullStr | An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| title_full_unstemmed | An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| title_short | An electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| title_sort | electrophoretic study of fetal mouse brain proteins after in vivo exposure to phenytoin and disulfiram |
| topic | Disulfiram - Toxicology Phenytoin - Toxicology Fetus - Effect of drugs on Teratogenic agents Disulfiram - Toxicology Phenytoin - toxicity Fetal proteins - drug effects Fetus - Drug effects Tera |
| url | http://hdl.handle.net/11427/27187 |
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