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Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector
Includes bibliographical references (leaves 89-95).
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Virology
2014
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| _version_ | 1867613143772954624 |
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| access_status_str | Open Access |
| author | Griffin, Sarah |
| author2 | Chapman, Rosamund |
| author_browse | Chapman, Rosamund Griffin, Sarah |
| author_facet | Chapman, Rosamund Griffin, Sarah |
| author_sort | Griffin, Sarah |
| collection | Thesis |
| description | Includes bibliographical references (leaves 89-95). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/2727 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:28.055Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Virology |
| publisherStr | Division of Virology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/2727 Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector Griffin, Sarah Chapman, Rosamund Medical Virology Includes bibliographical references (leaves 89-95). This study aimed to investigate whether the mycobacterial replicon may also play a role. The common mycobacterial replicon in episomal E. coli-mycobacterial shuttle vectors contains the truncated rap gene, encoding an auxiliary replication factor, and the repA and repB genes which code for essential replication proteins. Specific aspects of this typical mycobacterial replicon were identified as potential targets to improve stability and increase recombinant protein expression levels, and were modified accordingly. 2014-07-28T08:19:16Z 2014-07-28T08:19:16Z 2007 Master Thesis Masters MSc http://hdl.handle.net/11427/2727 eng application/pdf Division of Virology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medical Virology Griffin, Sarah Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector |
| thesis_degree_str | Master's |
| title | Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector |
| title_full | Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector |
| title_fullStr | Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector |
| title_full_unstemmed | Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector |
| title_short | Optimisation of the mycobacterial replicon of an E. coli-mycobacterial shuttle vector |
| title_sort | optimisation of the mycobacterial replicon of an e coli mycobacterial shuttle vector |
| topic | Medical Virology |
| url | http://hdl.handle.net/11427/2727 |
| work_keys_str_mv | AT griffinsarah optimisationofthemycobacterialrepliconofanecolimycobacterialshuttlevector |