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Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats
Includes abstract.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
MRC/UCT RU for Exercise and Sport Medicine
2014
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| _version_ | 1867613889565294592 |
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| access_status_str | Open Access |
| author | Goyaram, Veeraj |
| author2 | Ojuka, Edward O |
| author_browse | Goyaram, Veeraj Ojuka, Edward O |
| author_facet | Ojuka, Edward O Goyaram, Veeraj |
| author_sort | Goyaram, Veeraj |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/2751 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:43:19.442Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | MRC/UCT RU for Exercise and Sport Medicine |
| publisherStr | MRC/UCT RU for Exercise and Sport Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/2751 Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats Goyaram, Veeraj Ojuka, Edward O Exercise Science Includes abstract. Includes bibliographical references. Several studies have found that the expression of skeletal muscle glucose transporter 4 (GLUT4) is decreased by high fructose consumption but increased by exercise. However, the amounts of fructose used in these studies were extremely high and the effects of moderate feeding protocols are not known. While it is known that exercise enhances GLUT4 expression via increased histone H3 acetylation and binding of the myocyte enhancer factor- 2A (MEF2A) transcription factor to its binding domain on the Glut4 gene promoter, the impact of fructose consumption on this interaction has not been studied. Moreover, there is no direct evidence that an increase in MEF2 binding is due to increased accessibility of the MEF2 binding domain to transcription factors. This study tested the hypothesis that both exercise and high fructose consumption affect GLUT4 expression by altering the accessibility of the MEF2 binding domain on the Glut4 gene promoter via remodelling of chromatin in that region. Male Wistar rats (n=30) were randomly assigned to three dietary groups: a) standard Chow, b) Chow + 10% fructose drink and c) Chow + 10% maltodextrin drink. All rats had access to drinking water and chow ad libitum for a period of 13 days. In the last 6 days of the experiment 5 animals in each group performed 3 x 17 min daily bouts of intermittent swimming, with a load equivalent to 5% bodyweight attached to their tails. The remaining 5 rats from each group were untrained. Animals were fasted overnight on the last day of the experiment, anaesthetized and sacrificed on the morning of day 14. Triceps muscle were harvested and used: (a) for measurement of total GLUT4 content by western blot, (b) to obtain nuclei for assessment of accessibility of a 350bp region encompassing the MEF2 element on the Glut4 gene using nuclease digestion assay, and (c) to measure the acetylation of histones H3 and bound MEF2A in the region above using chromatin immunoprecipitation (ChIP) assay. Blood was also collected and assayed for fasting serum glucose, insulin and free fatty acids. 2014-07-28T08:23:35Z 2014-07-28T08:23:35Z 2013 Master Thesis Masters MScMed http://hdl.handle.net/11427/2751 eng application/pdf MRC/UCT RU for Exercise and Sport Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Exercise Science Goyaram, Veeraj Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats |
| thesis_degree_str | Master's |
| title | Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats |
| title_full | Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats |
| title_fullStr | Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats |
| title_full_unstemmed | Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats |
| title_short | Regulation of skeletal muscle glucose transporter 4 expression in fructose-fed exercised rats |
| title_sort | regulation of skeletal muscle glucose transporter 4 expression in fructose fed exercised rats |
| topic | Exercise Science |
| url | http://hdl.handle.net/11427/2751 |
| work_keys_str_mv | AT goyaramveeraj regulationofskeletalmuscleglucosetransporter4expressioninfructosefedexercisedrats |