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Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats
The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of ca...
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| Format: | Thesis |
| Language: | English |
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Division of Endocrinology and Diabetology
2018
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| _version_ | 1867613202949341184 |
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| access_status_str | Open Access |
| author | Shapiro, Brahm |
| author2 | Pimstone, Bernard L |
| author_browse | Pimstone, Bernard L Shapiro, Brahm |
| author_facet | Pimstone, Bernard L Shapiro, Brahm |
| author_sort | Shapiro, Brahm |
| collection | Thesis |
| description | The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/27657 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:24.523Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Endocrinology and Diabetology |
| publisherStr | Division of Endocrinology and Diabetology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/27657 Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats Shapiro, Brahm Pimstone, Bernard L Somatomedins Protein deficiency The studies presented in this thesis represent an attempt to characterise some aspects of the abnormalities of somatomedin physiology in protein-energy malnutrition. A suitable bioassay to study somatomedin was found in that of Van den Brande and Du Caju (1974) which makes use of uniform discs of cartilage punched from slices of immature porcine costal cartilage. The handling of the cartilage discs was made easy by the specially designed incubation rack. Preincubation increased sensitivity of the cartilage, and post incubation with radioactive tracer reduced the potential interference of varying sulphate content of assay samples. Serum samples were subjected to formal multipoint parallel line bioassay and data analysed by a computer programme designed to examine such assays. The bioassay was found to be sensitive to a concentration of serum of 5% and to have an index precision of less than 0.30 (and usually less than 0.20). 2018-03-15T07:38:00Z 2018-03-15T07:38:00Z 1978 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/27657 eng application/pdf Division of Endocrinology and Diabetology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Somatomedins Protein deficiency Shapiro, Brahm Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| thesis_degree_str | Doctoral |
| title | Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| title_full | Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| title_fullStr | Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| title_full_unstemmed | Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| title_short | Serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| title_sort | serum somatomedin and somatomedin generation by the perfused liver in protein malnourished rats |
| topic | Somatomedins Protein deficiency |
| url | http://hdl.handle.net/11427/27657 |
| work_keys_str_mv | AT shapirobrahm serumsomatomedinandsomatomedingenerationbytheperfusedliverinproteinmalnourishedrats |