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Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit
DNA profiling is routinely used in the forensic setting to identify individuals during criminal and medico-legal investigations. Its principle is based on the molecular analysis of DNA to produce a string of alpha-numeric characters which can be matched to a known reference sample. The use of allele...
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| Format: | Thesis |
| Language: | English |
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Division of Forensic Medicine and Toxicology
2018
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| _version_ | 1867613155272687616 |
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| access_status_str | Open Access |
| author | Nel, Lorraine |
| author2 | Heathfield, Laura J |
| author_browse | Heathfield, Laura J Nel, Lorraine |
| author_facet | Heathfield, Laura J Nel, Lorraine |
| author_sort | Nel, Lorraine |
| collection | Thesis |
| description | DNA profiling is routinely used in the forensic setting to identify individuals during criminal and medico-legal investigations. Its principle is based on the molecular analysis of DNA to produce a string of alpha-numeric characters which can be matched to a known reference sample. The use of allele frequencies from the background population aids the statistical interpretation of a match and can be used to calculate the random match probability. In South Africa, allele frequency data for the background population is currently limited, which can hinder the discriminatory value of DNA evidence, particularly when only a partial profile is obtained. Therefore, the aim of this study was to generate DNA allele frequency data for four South African population groups using the QIAGEN Investigator® 24PLEX GO! Kit, which has six markers for which data does not yet exist for the South African population. Full forensic DNA profiles were generated from 655 unrelated individuals from four population groups in South Africa: Black African (n = 172), Coloured (n = 195), Indian/Asian (n = 88) and White (n = 200). A 98% first time success rate was observed using the direct PCR approach. Allele frequencies were significantly different between all four population groups at three markers (D8S1179, D2S1338 and D2S441) after a Bonferroni correction (ρ < 0.001) and sixteen novel alleles were observed. Two genetic anomalies were observed, namely triallelic patterns at the TPOX marker (n = 9) and a null allele at amelogenin (n = 1). While the sample size for the Indian/Asian population group was limited in this study, the data generated here nevertheless prospects to contribute towards the data currently published for South Africa. This, in turn, will allow for more DNA markers to be analysed during forensic casework in South Africa, as the data for its statistical interpretation is now available. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/28054 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:38.662Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2018 |
| publishDateRange | 2018 |
| publishDateSort | 2018 |
| publisher | Division of Forensic Medicine and Toxicology |
| publisherStr | Division of Forensic Medicine and Toxicology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/28054 Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit Nel, Lorraine Heathfield, Laura J Biomedical Forensic Science DNA profiling is routinely used in the forensic setting to identify individuals during criminal and medico-legal investigations. Its principle is based on the molecular analysis of DNA to produce a string of alpha-numeric characters which can be matched to a known reference sample. The use of allele frequencies from the background population aids the statistical interpretation of a match and can be used to calculate the random match probability. In South Africa, allele frequency data for the background population is currently limited, which can hinder the discriminatory value of DNA evidence, particularly when only a partial profile is obtained. Therefore, the aim of this study was to generate DNA allele frequency data for four South African population groups using the QIAGEN Investigator® 24PLEX GO! Kit, which has six markers for which data does not yet exist for the South African population. Full forensic DNA profiles were generated from 655 unrelated individuals from four population groups in South Africa: Black African (n = 172), Coloured (n = 195), Indian/Asian (n = 88) and White (n = 200). A 98% first time success rate was observed using the direct PCR approach. Allele frequencies were significantly different between all four population groups at three markers (D8S1179, D2S1338 and D2S441) after a Bonferroni correction (ρ < 0.001) and sixteen novel alleles were observed. Two genetic anomalies were observed, namely triallelic patterns at the TPOX marker (n = 9) and a null allele at amelogenin (n = 1). While the sample size for the Indian/Asian population group was limited in this study, the data generated here nevertheless prospects to contribute towards the data currently published for South Africa. This, in turn, will allow for more DNA markers to be analysed during forensic casework in South Africa, as the data for its statistical interpretation is now available. 2018-05-14T12:26:14Z 2018-05-14T12:26:14Z 2018 Master Thesis Masters MPhil http://hdl.handle.net/11427/28054 eng application/pdf Division of Forensic Medicine and Toxicology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Biomedical Forensic Science Nel, Lorraine Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit |
| thesis_degree_str | Master's |
| title | Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit |
| title_full | Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit |
| title_fullStr | Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit |
| title_full_unstemmed | Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit |
| title_short | Constructing a DNA profile frequency database for South Africa using the Qiagen Investigator 24plex GO! Kit |
| title_sort | constructing a dna profile frequency database for south africa using the qiagen investigator 24plex go kit |
| topic | Biomedical Forensic Science |
| url | http://hdl.handle.net/11427/28054 |
| work_keys_str_mv | AT nellorraine constructingadnaprofilefrequencydatabaseforsouthafricausingtheqiageninvestigator24plexgokit |