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An immunohistochemical study of beta-catenin in HNPCC colon tumours
Includes bibliographical references (leaves 49-55).
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Division of Anatomical Pathology
2014
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| _version_ | 1867613150768005120 |
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| access_status_str | Open Access |
| author | Watkins, Jennifer G |
| author2 | Hall, Pauline de la Motte |
| author_browse | Hall, Pauline de la Motte Watkins, Jennifer G |
| author_facet | Hall, Pauline de la Motte Watkins, Jennifer G |
| author_sort | Watkins, Jennifer G |
| collection | Thesis |
| description | Includes bibliographical references (leaves 49-55). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/3087 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:34.243Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Anatomical Pathology |
| publisherStr | Division of Anatomical Pathology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/3087 An immunohistochemical study of beta-catenin in HNPCC colon tumours Watkins, Jennifer G Hall, Pauline de la Motte Anatomical Pathology Includes bibliographical references (leaves 49-55). Beta-catenin is normally complexed with adenomatous polyposis coli (APC) protein and E-cadherin adhesion molecule, and localized on the cell membrane. If APC/beta-catenin is disrupted, beta-catenin transfers into the nucleus, where it functions as a transcriptional activator, causing unregulated cell proliferation. The localisation of beta-catenin in H NPCC adenomas has not been studied but a shift in beta-catenin to the nucleus has been previously demonstrated in a range of col 0 recta I cancers, including those in HNPCC. The aim of the first part of the study was to determine whether there is a beta-catenin shift occurring as an early event in HNPCC tumours. Coded sections of tumours were immunohistochemically stained with antibody against beta-catenin and counterstained in haematoxylin. 14 HNPCC adenomas, 13 HNPCC carcinomas, 10 FAP adenomas, 10 FAP carcinomas, 10 sporadic adenomas and carcinomas and 10 juvenile polyps -three with dysplasia and seven without- were studied. A score was given for loss of membrane staining (0-1), presence of cytoplasmic staining (0-2) or nuclear staining (0- 2) and a total out of five obtained. An shift in beta-catenin was demonstrated at the adenoma phase in HNPCC. HNPCC, tumours were compared with sporadic tumours and a statistically significant similarity in prevalence of beta-catenin shift found in adenomas and carcinomas. The early shift in beta-catenin in HNPCC led to the second part of the study evaluating the "down-stream" effects of this shift in HNPCC tumours. TheHNPCC sections were immunohistochemically stained with E-cadherin, cmyc and cyclin 01. The results showed a positive correlation between Ecadherin loss, increased cyclin 01 and a shift in beta-catenin. No significant change in c-myc or correlation between c-myc and a shift of beta-catenin was found. In conclusion the study indicates that disruption of the APC/beta-catenin pathway plays a similar role in HNPCC tumours to that in sporadic tumours. A notable exeption is the effect on c-myc and further study is needed in this regard. 2014-07-28T14:51:48Z 2014-07-28T14:51:48Z 2004 Master Thesis Masters MMed http://hdl.handle.net/11427/3087 eng application/pdf Division of Anatomical Pathology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Anatomical Pathology Watkins, Jennifer G An immunohistochemical study of beta-catenin in HNPCC colon tumours |
| thesis_degree_str | Master's |
| title | An immunohistochemical study of beta-catenin in HNPCC colon tumours |
| title_full | An immunohistochemical study of beta-catenin in HNPCC colon tumours |
| title_fullStr | An immunohistochemical study of beta-catenin in HNPCC colon tumours |
| title_full_unstemmed | An immunohistochemical study of beta-catenin in HNPCC colon tumours |
| title_short | An immunohistochemical study of beta-catenin in HNPCC colon tumours |
| title_sort | immunohistochemical study of beta catenin in hnpcc colon tumours |
| topic | Anatomical Pathology |
| url | http://hdl.handle.net/11427/3087 |
| work_keys_str_mv | AT watkinsjenniferg animmunohistochemicalstudyofbetacatenininhnpcccolontumours AT watkinsjenniferg immunohistochemicalstudyofbetacatenininhnpcccolontumours |