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Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort
Includes abstract.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Human Genetics
2014
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| _version_ | 1867613306261340160 |
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| access_status_str | Open Access |
| author | Watson, Lauren |
| author2 | Greenberg, Jacquie |
| author_browse | Greenberg, Jacquie Watson, Lauren |
| author_facet | Greenberg, Jacquie Watson, Lauren |
| author_sort | Watson, Lauren |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/3108 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:00.978Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Human Genetics |
| publisherStr | Division of Human Genetics |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/3108 Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort Watson, Lauren Greenberg, Jacquie Wood, Matthew Human Genetics Includes abstract. Includes bibliographical references. Spinocerebellar ataxia type 7 (SCA7) is a dominantly-inherited neurodegenerative disease, resulting from a CAG trinucleotide repeat expansion in the ataxin-7 gene. The Ataxin-7 protein is known to play a role in transcriptional regulation through association with cellular histone acetylation complexes, and several studies have highlighted the role of transcriptional dysregulation, caused by the presence of mutant Ataxin-7, in the neuronal dysfunction that precedes the onset of disease symptoms.This study aimed to establish patient-derived cell models of SCA7, for use in the investigation of pathogenesis (with particular reference to transcriptional alterations), and in the evaluation of previously-developed therapies for the disease.The high prevalence of SCA7 in the South African population, as a result of a founder effect, makes this disease particularly amenable to allele-specific RNA interference (RNAi)-based therapy. Thus, this study also evaluated the feasibility of these cell models as a vehicle to test previously-developed RNAi therapeutics, using the alteration of expression of key transcripts as a phenotypic marker. SCA7 patient and control dermal fibroblasts were reprogrammed to pluripotency by retroviral transduction. The resultant induced pluripotent stem cell (iPSC) lines were characterised with respect to endogenous markers of pluripotency, differentiation capacity and transgene silencing. These cells were then subjected to neuronal differentiation, the success of which was confirmed by the expression of early neuronal markers. 2014-07-28T14:53:20Z 2014-07-28T14:53:20Z 2012 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/3108 eng application/pdf Division of Human Genetics Faculty of Health Sciences University of Cape Town |
| spellingShingle | Human Genetics Watson, Lauren Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort |
| thesis_degree_str | Doctoral |
| title | Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort |
| title_full | Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort |
| title_fullStr | Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort |
| title_full_unstemmed | Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort |
| title_short | Novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a South African patient cohort |
| title_sort | novel cell models for the study of spinocerebellar ataxia type 7 pathogenesis and therapy in a south african patient cohort |
| topic | Human Genetics |
| url | http://hdl.handle.net/11427/3108 |
| work_keys_str_mv | AT watsonlauren novelcellmodelsforthestudyofspinocerebellarataxiatype7pathogenesisandtherapyinasouthafricanpatientcohort |