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Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Division of Immunology
2014
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| _version_ | 1867613181718822912 |
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| access_status_str | Open Access |
| author | Barkhuizen, Mark |
| author2 | Brombacher, Frank |
| author_browse | Barkhuizen, Mark Brombacher, Frank |
| author_facet | Brombacher, Frank Barkhuizen, Mark |
| author_sort | Barkhuizen, Mark |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/3119 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:03.909Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Division of Immunology |
| publisherStr | Division of Immunology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/3119 Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection Barkhuizen, Mark Brombacher, Frank Magez, Sefan Immunology Includes bibliographical references. African trypanosomiasis encompasses diseases caused by pathogenic trypanosomes, infecting both humans and animals alike. To determine the immunological role of IL=12 family members in Trypanosoma brucei brucei, Trypanosoma evansi and Trypanosoma congolense infections, IL-12p35¯/¯, IL-12p40¯/¯ and IL-12p35¯/¯/p40¯/¯ mice were used. While the two latter mouse strains lack all IL-12 homologues, IL-12p35¯/¯ mice still produce IL-12p80 homodimers and IL-23. In infection with T.b. brucei and T.evansi; IL-12p35¯/¯, IL-12p40¯/¯ or IL-12p35¯/¯/p40¯/¯ mice were susceptible to both these pathogens, demonstrated by increased mortality compared to wild type C57BL/6 mice. The different IL-12 deficient mouse strains showed similar mortality kinetics, suggesting that IL-12p70 but not the IL-12p80 homodimer or IL-23 plays a crucial role in survival. Similarly, parasitemia control was reduced in the absence of IL-12p70. While plasma levels of IgM and IgG2c were similar between IL-12 deficient mice and wild type mice, IF-γ production. As IFN-γR¯/¯ mice were also highly susceptible to both T.b. brucei and T. evansi, IL-12p70-dependent IFN-γ production seems to be important mechanism involved in resistance against both these pathogens. 2014-07-28T14:54:13Z 2014-07-28T14:54:13Z 2008 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/3119 eng application/pdf Division of Immunology Faculty of Health Sciences University of Cape Town |
| spellingShingle | Immunology Barkhuizen, Mark Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection |
| thesis_degree_str | Doctoral |
| title | Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection |
| title_full | Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection |
| title_fullStr | Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection |
| title_full_unstemmed | Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection |
| title_short | Determination of the role of cytokines using gene deficient mice in African trypanosomiasis infection |
| title_sort | determination of the role of cytokines using gene deficient mice in african trypanosomiasis infection |
| topic | Immunology |
| url | http://hdl.handle.net/11427/3119 |
| work_keys_str_mv | AT barkhuizenmark determinationoftheroleofcytokinesusinggenedeficientmiceinafricantrypanosomiasisinfection |