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A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L
Using fresh papaya latex as the starting material, this thesis describes methods for the isolation and purification of three major proteinases by column adsorption chromatography; a comparative study of certain salient chemical and physical properties of these enzymes is presented. Reference is made...
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| Format: | Thesis |
| Language: | English |
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Division of Chemical Pathology
2020
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| _version_ | 1867613305549357056 |
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| access_status_str | Open Access |
| author | Skelton, Gerald S |
| author2 | Kench, J E |
| author_browse | Kench, J E Skelton, Gerald S |
| author_facet | Kench, J E Skelton, Gerald S |
| author_sort | Skelton, Gerald S |
| collection | Thesis |
| description | Using fresh papaya latex as the starting material, this thesis describes methods for the isolation and purification of three major proteinases by column adsorption chromatography; a comparative study of certain salient chemical and physical properties of these enzymes is presented. Reference is made to some conflicting results in the
literature concerning papain studies, and an effort has been made in this work, by further experimentation, to account for some of the discrepancies. A supplementary study of biochemical interest has been the collecting of latex from the same growing fruit at fixed time intervals : analysis of the samples shows the changes in the yields of latex and enzyme content during the six months that the fruit takes to ripen. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/31928 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:00.978Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Division of Chemical Pathology |
| publisherStr | Division of Chemical Pathology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/31928 A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L Skelton, Gerald S Kench, J E Hydrolases Using fresh papaya latex as the starting material, this thesis describes methods for the isolation and purification of three major proteinases by column adsorption chromatography; a comparative study of certain salient chemical and physical properties of these enzymes is presented. Reference is made to some conflicting results in the literature concerning papain studies, and an effort has been made in this work, by further experimentation, to account for some of the discrepancies. A supplementary study of biochemical interest has been the collecting of latex from the same growing fruit at fixed time intervals : analysis of the samples shows the changes in the yields of latex and enzyme content during the six months that the fruit takes to ripen. 2020-05-19T13:03:57Z 2020-05-19T13:03:57Z 1968 2020-04-15T09:15:44Z Doctoral Thesis Doctoral https://hdl.handle.net/11427/31928 eng application/pdf Division of Chemical Pathology Faculty of Health Sciences |
| spellingShingle | Hydrolases Skelton, Gerald S A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L |
| thesis_degree_str | Doctoral |
| title | A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L |
| title_full | A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L |
| title_fullStr | A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L |
| title_full_unstemmed | A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L |
| title_short | A biochemical study of some of the hydrolases to be found in the latex of the pawpaw, Carica papaya L |
| title_sort | biochemical study of some of the hydrolases to be found in the latex of the pawpaw carica papaya l |
| topic | Hydrolases |
| url | https://hdl.handle.net/11427/31928 |
| work_keys_str_mv | AT skeltongeralds abiochemicalstudyofsomeofthehydrolasestobefoundinthelatexofthepawpawcaricapapayal AT skeltongeralds biochemicalstudyofsomeofthehydrolasestobefoundinthelatexofthepawpawcaricapapayal |