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Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat
A sensitive and reproducible double antibody radioimmunoassay technique, requiring 50ul of unknown serum or plasma per assay tube, is described for use with 125I and rabbit anti-rat LH serum. The assay system was applied to the study of LH secretion in rats under both normal and experimentally manip...
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| Format: | Thesis |
| Language: | English |
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Division of Medical Biochemistry
2020
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| _version_ | 1867613206200975360 |
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| access_status_str | Open Access |
| author | Querido, David |
| author2 | Beardwoor, C J |
| author_browse | Beardwoor, C J Querido, David |
| author_facet | Beardwoor, C J Querido, David |
| author_sort | Querido, David |
| collection | Thesis |
| description | A sensitive and reproducible double antibody radioimmunoassay technique, requiring 50ul of unknown serum or plasma per assay tube, is described for use with 125I and rabbit anti-rat LH serum. The assay system was applied to the study of LH secretion in rats under both normal and experimentally manipulated conditions. Particular attention was focussed upon comparison of circulating LH levels in conscious, unstressed animals with those in anaesthetized animals, with or without surgical stress. Thereafter, the effects of acoustic stimulation and of exogenous LRH administration were studied in conscious and anaesthetized animals. Urethane anaesthesia exerted a profound effect upon the LR-secretory response to exogenous LRH in male rats. Available evidence suggests that the blood sampling
method, surgical stress and anaesthesia are each capable of significantly influencing LH secretion, thereby emphasizing the value of studies using conscious, unstressed animals. While a direct effect of urethane on the pituitary gland cannot be excluded, attention is drawn to the possible mediation of a urethane-sensitive inhibitory influence in the mechanism controlling LH secretion in the rat. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/31974 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:27.580Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2020 |
| publishDateRange | 2020 |
| publishDateSort | 2020 |
| publisher | Division of Medical Biochemistry |
| publisherStr | Division of Medical Biochemistry |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/31974 Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat Querido, David Beardwoor, C J Radioimmunoassay hormone secretion rat A sensitive and reproducible double antibody radioimmunoassay technique, requiring 50ul of unknown serum or plasma per assay tube, is described for use with 125I and rabbit anti-rat LH serum. The assay system was applied to the study of LH secretion in rats under both normal and experimentally manipulated conditions. Particular attention was focussed upon comparison of circulating LH levels in conscious, unstressed animals with those in anaesthetized animals, with or without surgical stress. Thereafter, the effects of acoustic stimulation and of exogenous LRH administration were studied in conscious and anaesthetized animals. Urethane anaesthesia exerted a profound effect upon the LR-secretory response to exogenous LRH in male rats. Available evidence suggests that the blood sampling method, surgical stress and anaesthesia are each capable of significantly influencing LH secretion, thereby emphasizing the value of studies using conscious, unstressed animals. While a direct effect of urethane on the pituitary gland cannot be excluded, attention is drawn to the possible mediation of a urethane-sensitive inhibitory influence in the mechanism controlling LH secretion in the rat. 2020-05-22T13:41:23Z 2020-05-22T13:41:23Z 1975 2020-04-14T08:19:02Z Master Thesis Masters https://hdl.handle.net/11427/31974 eng application/pdf Division of Medical Biochemistry Faculty of Health Sciences |
| spellingShingle | Radioimmunoassay hormone secretion rat Querido, David Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| thesis_degree_str | Master's |
| title | Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| title_full | Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| title_fullStr | Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| title_full_unstemmed | Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| title_short | Applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| title_sort | applications of a radioimmunoassay technique to the study of luteinizing hormone secretion in the rat |
| topic | Radioimmunoassay hormone secretion rat |
| url | https://hdl.handle.net/11427/31974 |
| work_keys_str_mv | AT queridodavid applicationsofaradioimmunoassaytechniquetothestudyofluteinizinghormonesecretionintherat |