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The role of Surfacant Protein D in the control of human helminth infections
Lung produced surfactant protein D (SP-D) is essential for both homeostasis and as an innate immune opsonin. In the project presented here, we aimed to translate data recently published by our group, which demonstrated that SP-D contributes to protection against murine parasitic nematode infections,...
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| Format: | Thesis |
| Language: | English |
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Division of Immunology
2021
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| _version_ | 1867613164387958784 |
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| access_status_str | Open Access |
| author | Baker, Zoe |
| author2 | Horsnell, William |
| author_browse | Baker, Zoe Horsnell, William |
| author_facet | Horsnell, William Baker, Zoe |
| author_sort | Baker, Zoe |
| collection | Thesis |
| description | Lung produced surfactant protein D (SP-D) is essential for both homeostasis and as an innate immune opsonin. In the project presented here, we aimed to translate data recently published by our group, which demonstrated that SP-D contributes to protection against murine parasitic nematode infections, to human work. In the first part of this study, we determined whether individuals exposed to helminths have altered serum SP-D in comparison to unexposed individuals, through analysis (ELISA and Western Blot) of bio banked samples in 2 clinical cohorts from South Africa. Secondly, we aimed to identify if SP-D influences the magnitude of anti-nematode responses in human immune cells (type 2 innate lymphoid cells, monocytes and macrophages) through in vitro cell work and flow cytometry. Our findings indicated an association between serum SP-D and exposure to helminths that have a lung migration stage as part of their life cycle (Ascaris spp and Toxocara spp). Furthermore, in vitro analysis demonstrated that human immune cells primed with SP-D might have an altered response to helminth antigen. These findings point toward the need for further investigation into the novel role of SP-D in the control of human helminth infections in the context of immune physiology, as a biomarker and eventually treatment option. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/32585 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:47.142Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2021 |
| publishDateRange | 2021 |
| publishDateSort | 2021 |
| publisher | Division of Immunology |
| publisherStr | Division of Immunology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/32585 The role of Surfacant Protein D in the control of human helminth infections Baker, Zoe Horsnell, William Clinical Science and Immunology Lung produced surfactant protein D (SP-D) is essential for both homeostasis and as an innate immune opsonin. In the project presented here, we aimed to translate data recently published by our group, which demonstrated that SP-D contributes to protection against murine parasitic nematode infections, to human work. In the first part of this study, we determined whether individuals exposed to helminths have altered serum SP-D in comparison to unexposed individuals, through analysis (ELISA and Western Blot) of bio banked samples in 2 clinical cohorts from South Africa. Secondly, we aimed to identify if SP-D influences the magnitude of anti-nematode responses in human immune cells (type 2 innate lymphoid cells, monocytes and macrophages) through in vitro cell work and flow cytometry. Our findings indicated an association between serum SP-D and exposure to helminths that have a lung migration stage as part of their life cycle (Ascaris spp and Toxocara spp). Furthermore, in vitro analysis demonstrated that human immune cells primed with SP-D might have an altered response to helminth antigen. These findings point toward the need for further investigation into the novel role of SP-D in the control of human helminth infections in the context of immune physiology, as a biomarker and eventually treatment option. 2021-01-20T08:41:50Z 2021-01-20T08:41:50Z 2020 2020-12-23T09:21:37Z Master Thesis Masters MSc http://hdl.handle.net/11427/32585 eng application/pdf Division of Immunology Faculty of Health Sciences |
| spellingShingle | Clinical Science and Immunology Baker, Zoe The role of Surfacant Protein D in the control of human helminth infections |
| thesis_degree_str | Master's |
| title | The role of Surfacant Protein D in the control of human helminth infections |
| title_full | The role of Surfacant Protein D in the control of human helminth infections |
| title_fullStr | The role of Surfacant Protein D in the control of human helminth infections |
| title_full_unstemmed | The role of Surfacant Protein D in the control of human helminth infections |
| title_short | The role of Surfacant Protein D in the control of human helminth infections |
| title_sort | role of surfacant protein d in the control of human helminth infections |
| topic | Clinical Science and Immunology |
| url | http://hdl.handle.net/11427/32585 |
| work_keys_str_mv | AT bakerzoe theroleofsurfacantproteindinthecontrolofhumanhelminthinfections AT bakerzoe roleofsurfacantproteindinthecontrolofhumanhelminthinfections |