Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Cardiac mitochondrial respiration in two rodent models of obesity
Includes bibliographical references (leaves 95-107)
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Medicine
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613611682168832 |
|---|---|
| access_status_str | Open Access |
| author | Chan, Wing Yin Anna |
| author2 | Essop, Faadiel |
| author_browse | Chan, Wing Yin Anna Essop, Faadiel |
| author_facet | Essop, Faadiel Chan, Wing Yin Anna |
| author_sort | Chan, Wing Yin Anna |
| collection | Thesis |
| description | Includes bibliographical references (leaves 95-107) |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/3371 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:38:54.432Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Medicine |
| publisherStr | Department of Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/3371 Cardiac mitochondrial respiration in two rodent models of obesity Chan, Wing Yin Anna Essop, Faadiel Medicine Includes bibliographical references (leaves 95-107) Obesity is a major contributor to the global burden of disease and is closely associated with the development of type II diabetes. Recent studies have demonstrated that increased circulating free fatty acid (FFA) levels may have detrimental effects on the diabetic heart. In this study, we hypothesized that with obesity and obesity-induced insulin resistance/type II diabetes, increased FFA supply decreases cardiac mitochondrial bioenergetic capacity. Furthermore, we also hypothesized that females possess innate cardioprotective programs that will result in enhanced bioenergetic capacity compared to males. We examined our hypothesis employing two rodent models i.e. a) a rat model of diet-induced obesity and b) a transgenic (leptin receptor deficient) mouse model of obesity-induced type II diabetes. For the diabetic mouse model, we determined cardiac mitochondrial respiratory function in an age-dependent (10-12, 18-20 and 55-56 weeks) and gender-dependent (male versus female) manner. We found impaired mitochondrial respiratory capacity in obese rats in baseline and when isolated mitochondria were stressed by anoxia-reoxygenation. We speculate that this may be dure to reduced expression of mitochondrial respiratory chain complexes in the insulin resistant rat heart. For the mouse model and type II diabetes we found increased respiratory capacity at 10-12 weeks, thought to respresent the stage of metabolic syndrome, with no evidence of oxygen wastage or reduction of respiratory capacity. However, 18-20 week-old obese mice were unable to increase respiratory capacity. We also found increased mitochondrial ultrastructural damage and intracellular lipid accumulation in 18-20 week-old diabetic mouse hearts. We propose that this occurs as a result of a mismatch between increased FA uptake and decreased FA oxidative capacity. 2014-07-29T09:02:56Z 2014-07-29T09:02:56Z 2006 Master Thesis Masters MSc http://hdl.handle.net/11427/3371 eng application/pdf Department of Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medicine Chan, Wing Yin Anna Cardiac mitochondrial respiration in two rodent models of obesity |
| thesis_degree_str | Master's |
| title | Cardiac mitochondrial respiration in two rodent models of obesity |
| title_full | Cardiac mitochondrial respiration in two rodent models of obesity |
| title_fullStr | Cardiac mitochondrial respiration in two rodent models of obesity |
| title_full_unstemmed | Cardiac mitochondrial respiration in two rodent models of obesity |
| title_short | Cardiac mitochondrial respiration in two rodent models of obesity |
| title_sort | cardiac mitochondrial respiration in two rodent models of obesity |
| topic | Medicine |
| url | http://hdl.handle.net/11427/3371 |
| work_keys_str_mv | AT chanwingyinanna cardiacmitochondrialrespirationintworodentmodelsofobesity |