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Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection
Includes abstract.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Medicine
2014
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| _version_ | 1867613224287862784 |
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| access_status_str | Open Access |
| author | Einhorn, Andrew |
| author2 | Brombacher, Frank |
| author_browse | Brombacher, Frank Einhorn, Andrew |
| author_facet | Brombacher, Frank Einhorn, Andrew |
| author_sort | Einhorn, Andrew |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/3380 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:44.899Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Medicine |
| publisherStr | Department of Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/3380 Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection Einhorn, Andrew Brombacher, Frank Medicine Includes abstract. Includes bibliographical references. This thesis is composed of two parts. The first is an investigation of the role of B-effector cells in the course of murine Leishmania Major (L. major). The second is a bioinformatic analysis of microarray slides of activated CD4 T-cells from L. major infected BALB/c and C57BL/6 mice in order to investigate the genetic determinants of their divergent disease phenotypes. Overall this thesis is an investigation of the role of B-and T-lymphocytes during the course of L. major infection in mice. B-cells are not traditionally thought to play a role in the pathogenesis of Leishmania major infection. It is well documented that T-helper 1(Th1) and T-helper 2 (Th2) responses lead to resistance and susceptiblity to L. major respectively. Recent studies have now shown that B-cells are capable to producing key T-cell differentiating cytokines such as IL-4 and IFN-γ. More specifically, two new B-cell populations have been identified: B effector 1 (Be1) and B effector 2 (Be2) cells, which produce IFN-γ and IL-4 respectively. Using mice lacking the IL-4Rα on B-cells, and thus incapble of producing Be2 cells, we investigate the contribution of Be2 cells to BALB/c susceptibility. We confirm that the delection of the IL-4Rα on B-cells renders the previously susceptible BALB/c mice resistant to L. major strain LV39 and further confirm the phenotype using the more the virulent IL81 strain. We demonstrate that mice lacking Be2 cells exhibit a dimished Th2 response and an enhanced Th1 response, suggesting that B-cells, in addition to their role in antibody production, may also play a role in shaping T-helper responses in vivo. In the case of L.major, this provides evidence for a novel link between B cells and the cellular immune response. 2014-07-29T09:03:20Z 2014-07-29T09:03:20Z 2013 Master Thesis Masters MSc http://hdl.handle.net/11427/3380 eng application/pdf Department of Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medicine Einhorn, Andrew Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection |
| thesis_degree_str | Master's |
| title | Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection |
| title_full | Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection |
| title_fullStr | Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection |
| title_full_unstemmed | Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection |
| title_short | Investigating the role of B and T lymphocytes in the course of murine Leishmania Major infection |
| title_sort | investigating the role of b and t lymphocytes in the course of murine leishmania major infection |
| topic | Medicine |
| url | http://hdl.handle.net/11427/3380 |
| work_keys_str_mv | AT einhornandrew investigatingtheroleofbandtlymphocytesinthecourseofmurineleishmaniamajorinfection |