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Studies on human protoporphyrinogen oxidase
Bibliography: p. 131-170.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Medicine
2014
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| _version_ | 1867613330478202880 |
|---|---|
| access_status_str | Open Access |
| author | Maneli, Mbulelo H |
| author2 | Meissner, Peter |
| author_browse | Maneli, Mbulelo H Meissner, Peter |
| author_facet | Meissner, Peter Maneli, Mbulelo H |
| author_sort | Maneli, Mbulelo H |
| collection | Thesis |
| description | Bibliography: p. 131-170. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/3424 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:25.395Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Medicine |
| publisherStr | Department of Medicine |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/3424 Studies on human protoporphyrinogen oxidase Maneli, Mbulelo H Meissner, Peter Corrigall, Anne Medicine Bibliography: p. 131-170. This study examines the effects of various protoporphyrinogen oxidase mutations responsible for variegate porphyria, the role of the arginine-59 residue, and the glycines in the conserved flavin binding site, in catalysis and/or cofactor binding. Wild type recombinant human protoporphyrinogen oxidase and a selection of both naturally occurring and self-designed mutants were generated, expresses and purified. The self designed mutants included a conservative and two non-conservative arginine-59 replacements, and substitution of glycine residues at positions 9, 11, and 14 by alanine. The expression and purification for all protoporphyrinogen oxidases was optimised, enabling their purification to homogeneity by single step metal affinity chromatography. 2014-07-29T09:07:12Z 2014-07-29T09:07:12Z 2002 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/3424 eng application/pdf Department of Medicine Faculty of Health Sciences University of Cape Town |
| spellingShingle | Medicine Maneli, Mbulelo H Studies on human protoporphyrinogen oxidase |
| thesis_degree_str | Doctoral |
| title | Studies on human protoporphyrinogen oxidase |
| title_full | Studies on human protoporphyrinogen oxidase |
| title_fullStr | Studies on human protoporphyrinogen oxidase |
| title_full_unstemmed | Studies on human protoporphyrinogen oxidase |
| title_short | Studies on human protoporphyrinogen oxidase |
| title_sort | studies on human protoporphyrinogen oxidase |
| topic | Medicine |
| url | http://hdl.handle.net/11427/3424 |
| work_keys_str_mv | AT manelimbuleloh studiesonhumanprotoporphyrinogenoxidase |