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Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings

Microbially Induced Calcite Precipitation (MICP) is an emerging bio-mediated technology which has been successfully applied in soil improvement research. MICP uses the enzyme urease produced from bacteria to breakdown urea into carbonate ions. These carbonate ions combine with free calcium ions to f...

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Main Author: Hyde, Rhonda
Other Authors: Nolutshungu, Lita
Format: Thesis
Language:English
Published: Department of Civil Engineering 2023
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access_status_str Open Access
author Hyde, Rhonda
author2 Nolutshungu, Lita
author_browse Hyde, Rhonda
Nolutshungu, Lita
author_facet Nolutshungu, Lita
Hyde, Rhonda
author_sort Hyde, Rhonda
collection Thesis
description Microbially Induced Calcite Precipitation (MICP) is an emerging bio-mediated technology which has been successfully applied in soil improvement research. MICP uses the enzyme urease produced from bacteria to breakdown urea into carbonate ions. These carbonate ions combine with free calcium ions to form calcium carbonate, which acts as a bio-cement. MICP presents a unique, sustainable soil improvement solution to the pressing issues resulting from tailings impoundment failures. It has shown potential through increasing shear strength and decreasing porosity in soils. However, MICP applications in soil improvement outside erosion mitigation in granular soils remain limited. This is similar to the limited use of injection treatment, in comparison to the more prevalent spraying and surface percolation in MICP applications. This research focused on the efficacy of the developed injection procedure for administering the MICP treatment to increase shear strength and decrease porosity in sand, clay and gold tailings at greater depths and evaluating its feasibility. By determining the efficacy and significance of the treatment in improving the geotechnical characteristics of the soil samples, the methodology can be evaluated for its application as a soil improvement technique. Results showed successful cementation of the particles of the soils tested with an increase in cohesion of 7.7% and 23.1% for clay, and tailings respectively and an infinite increase in the apparent cohesion of sand from 0 to 20kPa. The response to MICP treatment in terms of the angle of internal friction were inconclusive, where a decrease was observed across the board. This was attributed to complex stress-strain behaviour as well as the particle morphology. A decrease in porosity of approximately 26% in clay and 8% in tailings was observed, whilst sand had an increase of approximately 3%. The increase in porosity in sand was identified as a result of the erosion of the coarse uncemented particles during treatment. The results emphasised the greater success of MICP treatment in more granular soils, with sand achieving the greatest improvement with regard to the apparent cohesion and particle density. Characteristically, the particle sizes of the gold tailings fell between the fine clay and the coarse sand which was reflected in the response of the gold tailings to treatment. Overall, sand had the greatest increase in shear strength, followed by the gold tailings and lastly the clay. The gold tailings contained a higher percentage of fines than the sand, illustrating the limitation of MICP applications in fine grained soils. However, the predominant coarse fraction allowed for an overall increase in the shear strength parameters in the gold tailings. An evaluation of the feasibility shows that the methods provide a scalable soil improvement technique in stabilisation applications in contrast to existing MICP surface treatments in sands. In clays and tailings however, interactions of heavy metals with the microbial community as well as the particle size limit the efficacy of MICP. In conclusion, MICP is found to be a feasible soil improvement technique in stabilising gold tailings with the consideration of the impact of heavy metals and the particle size on the efficacy of the treatment.
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institution University of Cape Town (South Africa)
language eng
last_indexed 2026-06-10T12:33:28.738Z
license_str Not specified — see source repository
provenance_str_mv Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository
publishDate 2023
publishDateRange 2023
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spelling oai:open.uct.ac.za:11427/36926 Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings Hyde, Rhonda Nolutshungu, Lita Randall, Dyllon Geotechnical Engineering Microbially Induced Calcite Precipitation (MICP) is an emerging bio-mediated technology which has been successfully applied in soil improvement research. MICP uses the enzyme urease produced from bacteria to breakdown urea into carbonate ions. These carbonate ions combine with free calcium ions to form calcium carbonate, which acts as a bio-cement. MICP presents a unique, sustainable soil improvement solution to the pressing issues resulting from tailings impoundment failures. It has shown potential through increasing shear strength and decreasing porosity in soils. However, MICP applications in soil improvement outside erosion mitigation in granular soils remain limited. This is similar to the limited use of injection treatment, in comparison to the more prevalent spraying and surface percolation in MICP applications. This research focused on the efficacy of the developed injection procedure for administering the MICP treatment to increase shear strength and decrease porosity in sand, clay and gold tailings at greater depths and evaluating its feasibility. By determining the efficacy and significance of the treatment in improving the geotechnical characteristics of the soil samples, the methodology can be evaluated for its application as a soil improvement technique. Results showed successful cementation of the particles of the soils tested with an increase in cohesion of 7.7% and 23.1% for clay, and tailings respectively and an infinite increase in the apparent cohesion of sand from 0 to 20kPa. The response to MICP treatment in terms of the angle of internal friction were inconclusive, where a decrease was observed across the board. This was attributed to complex stress-strain behaviour as well as the particle morphology. A decrease in porosity of approximately 26% in clay and 8% in tailings was observed, whilst sand had an increase of approximately 3%. The increase in porosity in sand was identified as a result of the erosion of the coarse uncemented particles during treatment. The results emphasised the greater success of MICP treatment in more granular soils, with sand achieving the greatest improvement with regard to the apparent cohesion and particle density. Characteristically, the particle sizes of the gold tailings fell between the fine clay and the coarse sand which was reflected in the response of the gold tailings to treatment. Overall, sand had the greatest increase in shear strength, followed by the gold tailings and lastly the clay. The gold tailings contained a higher percentage of fines than the sand, illustrating the limitation of MICP applications in fine grained soils. However, the predominant coarse fraction allowed for an overall increase in the shear strength parameters in the gold tailings. An evaluation of the feasibility shows that the methods provide a scalable soil improvement technique in stabilisation applications in contrast to existing MICP surface treatments in sands. In clays and tailings however, interactions of heavy metals with the microbial community as well as the particle size limit the efficacy of MICP. In conclusion, MICP is found to be a feasible soil improvement technique in stabilising gold tailings with the consideration of the impact of heavy metals and the particle size on the efficacy of the treatment. 2023-02-15T11:31:53Z 2023-02-15T11:31:53Z 2022 2023-02-15T11:24:04Z Master Thesis Masters MSc http://hdl.handle.net/11427/36926 eng application/pdf Department of Civil Engineering Faculty of Engineering and the Built Environment
spellingShingle Geotechnical Engineering
Hyde, Rhonda
Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings
thesis_degree_str Master's
title Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings
title_full Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings
title_fullStr Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings
title_full_unstemmed Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings
title_short Investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand, clay and gold tailings
title_sort investigating the feasibility of implementing microbially induced calcite precipitation to stabilize sand clay and gold tailings
topic Geotechnical Engineering
url http://hdl.handle.net/11427/36926
work_keys_str_mv AT hyderhonda investigatingthefeasibilityofimplementingmicrobiallyinducedcalciteprecipitationtostabilizesandclayandgoldtailings