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The primary structure of histone H2B from the mollusc Patella granatina
Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2023
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| _version_ | 1867613256441397248 |
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| access_status_str | Open Access |
| author | Van Helden, Paul David |
| author2 | Von Holt, Claus |
| author_browse | Van Helden, Paul David Von Holt, Claus |
| author_facet | Von Holt, Claus Van Helden, Paul David |
| author_sort | Van Helden, Paul David |
| collection | Thesis |
| description | Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/38915 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:15.376Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2023 |
| publishDateRange | 2023 |
| publishDateSort | 2023 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/38915 The primary structure of histone H2B from the mollusc Patella granatina Van Helden, Paul David Von Holt, Claus Biochemistry Histones H2B were isolated from the gonads of a mollusc (Patella granatina) and from chicken (Gallus domesticus), crocodile (Crocodylus niloticus) and amphibian (Xenopus laevis) erythrocytes. The H2B's were purified by ion-exchange and gel exclusion chromatography. The complete primary structure of the mollusc hi stone H2B t 11 has been deduced from the sequences established of pa e a adjoining and overlapping peptides by the Edman degradation procedure. The partial structure of H2B from chicken erythrocytes (87 residues), crocodile erythrocytes (75 residues) and Xenopus erythrocytes (63 residues) was also established. The amino acid sequences are compared to those of other histones H2B. The effect of mutations on the predicted secondary structure of histone H2B is considered. 2023-09-27T14:06:04Z 2023-09-27T14:06:04Z 1978 2023-09-27T14:05:41Z Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/38915 eng application/pdf Department of Molecular and Cell Biology Faculty of Science |
| spellingShingle | Biochemistry Van Helden, Paul David The primary structure of histone H2B from the mollusc Patella granatina |
| thesis_degree_str | Doctoral |
| title | The primary structure of histone H2B from the mollusc Patella granatina |
| title_full | The primary structure of histone H2B from the mollusc Patella granatina |
| title_fullStr | The primary structure of histone H2B from the mollusc Patella granatina |
| title_full_unstemmed | The primary structure of histone H2B from the mollusc Patella granatina |
| title_short | The primary structure of histone H2B from the mollusc Patella granatina |
| title_sort | primary structure of histone h2b from the mollusc patella granatina |
| topic | Biochemistry |
| url | http://hdl.handle.net/11427/38915 |
| work_keys_str_mv | AT vanheldenpauldavid theprimarystructureofhistoneh2bfromthemolluscpatellagranatina AT vanheldenpauldavid primarystructureofhistoneh2bfromthemolluscpatellagranatina |