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The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants
The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blo...
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2024
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| _version_ | 1867613148409757696 |
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| access_status_str | Open Access |
| author | Garwe, Dahlia |
| author2 | Thomson, Jennifer |
| author_browse | Garwe, Dahlia Thomson, Jennifer |
| author_facet | Thomson, Jennifer Garwe, Dahlia |
| author_sort | Garwe, Dahlia |
| collection | Thesis |
| description | The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/40101 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:31.816Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2024 |
| publishDateRange | 2024 |
| publishDateSort | 2024 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/40101 The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants Garwe, Dahlia Thomson, Jennifer Mundree, Sagadevan Molecular and Cell Biology The strategy of "complementation by functional sufficiency" was used to isolate a cDNA, designated XVSAP1, from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. 2024-07-02T09:26:48Z 2024-07-02T09:26:48Z 2003 2024-07-01T09:41:20Z Thesis / Dissertation Doctoral PhD http://hdl.handle.net/11427/40101 eng application/pdf Department of Molecular and Cell Biology Faculty of Science |
| spellingShingle | Molecular and Cell Biology Garwe, Dahlia The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants |
| thesis_degree_str | Doctoral |
| title | The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants |
| title_full | The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants |
| title_fullStr | The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants |
| title_full_unstemmed | The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants |
| title_short | The characterization of XVSAP1: a gene isolated from the resurrection plant Xerophyta viscosa Baker, and its expression in transgenic plants |
| title_sort | characterization of xvsap1 a gene isolated from the resurrection plant xerophyta viscosa baker and its expression in transgenic plants |
| topic | Molecular and Cell Biology |
| url | http://hdl.handle.net/11427/40101 |
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