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Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity
Includes bibliographical references (leaves 126-140).
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613177287540736 |
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| access_status_str | Open Access |
| author | Bamuamba, Kapinga Benoit |
| author2 | Meyers, Paul |
| author_browse | Bamuamba, Kapinga Benoit Meyers, Paul |
| author_facet | Meyers, Paul Bamuamba, Kapinga Benoit |
| author_sort | Bamuamba, Kapinga Benoit |
| collection | Thesis |
| description | Includes bibliographical references (leaves 126-140). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4241 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:58.458Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4241 Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity Bamuamba, Kapinga Benoit Meyers, Paul Gammon, David W Franca, Marie-Geneviève Dijoux Cell Biology Includes bibliographical references (leaves 126-140). In our search for new anti-tuberculosis lead molecules, five medicinal plant species, Olea capensis (L.l, Tulbaghia alliacea (L.), Inula graveolens (L.), Leyssera gnaphaloides (L.), and Buddleja saligna (L.) were collected in Cape Town and surrounding area and investigated for antimycobacterial activity following report of their therapeutic use in traditional medicine to treat infectious diseases such as tuberculosis. A bioassay guided fractionation of the acetone/water (4:1) crude extracts of O. capensis (leaves) and T. alliacea (rhizomes) showed no activity against Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 252923, and Mycobacterium aurum A+. In contrast, the orgamc fractions (hexane, dichloromethane) of the acetone/water (4: 1) crude extracts of 1. graveolens, L. gnaphaloides, and B. saligna exhibited significant activity against M. tuberculosis H37Rv, M. avium 25291, M. microti ATCC 19422, and M. scrofulaceum ATCC 19987. The isolation and structure determination of the bioactive led to the identification of pentacyclic triterpenoids, ursolic acid (UA) and oleanolic acid as major antitubercular constituents of B. saligna, L. gnaphaloides, and 1. graveolens. The in vitro cytotoxicity assays of the isolated bioactive constituents showed no cytotoxicity against Chinese Hamster Ovarian (CHO) cells line. Subsequently, given the pharmaceutical value of the above finding, a survey on structure activity of pentacyclic triterpenoids was conducted. It was was found, for instance that selective substitutions at C-3 and/or C-28 and the double bond at UA, OA and betulinic and (1) BA) were made in order to improve anti-tumour and anti-HIV activity. However, thought a great number of modified bioactive pentacyclic triterpenoids is reported, none was tested against Mtb. Therefore, this study also explored a new synthetic route (scheme 1) toward a generation of (5), which may allow improving antitubercular, anti-HIV or anti-tumour activity, and/or specificity. 2014-07-30T17:35:33Z 2014-07-30T17:35:33Z 2006 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/4241 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Bamuamba, Kapinga Benoit Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity |
| thesis_degree_str | Doctoral |
| title | Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity |
| title_full | Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity |
| title_fullStr | Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity |
| title_full_unstemmed | Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity |
| title_short | Pharmacognostic study of 5 medicinal plant species from Western Cape Province (South Africa) for anti-tubercular activity |
| title_sort | pharmacognostic study of 5 medicinal plant species from western cape province south africa for anti tubercular activity |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4241 |
| work_keys_str_mv | AT bamuambakapingabenoit pharmacognosticstudyof5medicinalplantspeciesfromwesterncapeprovincesouthafricaforantitubercularactivity |