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The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation
Bibliography: leaves 165-195.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613205147156480 |
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| access_status_str | Open Access |
| author | Dube, Thabani |
| author2 | Thomson, Jennifer Ann |
| author_browse | Dube, Thabani Thomson, Jennifer Ann |
| author_facet | Thomson, Jennifer Ann Dube, Thabani |
| author_sort | Dube, Thabani |
| collection | Thesis |
| description | Bibliography: leaves 165-195. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4255 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:26.116Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4255 The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation Dube, Thabani Thomson, Jennifer Ann Bibliography: leaves 165-195. The transfer of T-DNA from Agrobacterium tumefaciens to eukaryotic cells is on of the most extensively studied examples of interkingdom DNA transfer. This transfer system has revolutionized plant molecular biology to the extent that the introduction of foreign genes into plant genomes is now a basic technique. Apart from the finding that a defined segment (T-DNA) of the A. tumefaciens Ti plasmid can be transferred to plant DNA, the discovery that the mobilization functions of the IncQ plasmid, RSF1010, can mediate its transfer from A. tumefaciens into plant cells as well between Gram negative bacteria has generated considerable interest. The mobilization functions of RSF1010 substitute for the requirement of the 25-bp border T-DNA sequences of A. tumefaciens. In this thesis I investigated the ability of another plasmid, pTF-FC2, to transfer genes to plants. 2014-07-30T17:36:51Z 2014-07-30T17:36:51Z 2002 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/4255 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Dube, Thabani The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation |
| thesis_degree_str | Doctoral |
| title | The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation |
| title_full | The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation |
| title_fullStr | The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation |
| title_full_unstemmed | The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation |
| title_short | The use of the broad-host-range plasmid, pTF-FC2, in Agrobacterium tumefaciens mediated plant transformation |
| title_sort | use of the broad host range plasmid ptf fc2 in agrobacterium tumefaciens mediated plant transformation |
| url | http://hdl.handle.net/11427/4255 |
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