Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa
Includes bibliographical references (leaves 59-71).
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
|
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613206380281856 |
|---|---|
| access_status_str | Open Access |
| author | Felix, Mba Medie |
| author2 | Rafudeen, Suhail |
| author_browse | Felix, Mba Medie Rafudeen, Suhail |
| author_facet | Rafudeen, Suhail Felix, Mba Medie |
| author_sort | Felix, Mba Medie |
| collection | Thesis |
| description | Includes bibliographical references (leaves 59-71). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4261 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:27.580Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4261 Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa Felix, Mba Medie Rafudeen, Suhail Farrant, Jill M Includes bibliographical references (leaves 59-71). A number of genes up-regulated in response to abiotic stress have been isolated from X. viscosa by various methods, one of which includes differential screening of cDNA libraries. Of these genes, a large number are undescribed, with no identity to known plant genes. Two such genes, XvRG6 and XvRF17, isolated from a X. viscosa cDNA library have been partially characterised. Southern blot analysis confirmed the presence of both XvRG6 and XvRF17 genes in the X. viscosa genome. In silico analyses predicted XvRG6 to be proline-rich protein, since it possesses many of the features common to PRP such as a signal peptide, proline repeats, a cysteine residue and possible phosphorylation sites. It also possesses a tyrosine residue present in some of the repeats, and this residue is belivied to play a role in protecting the plant against environmental stresses. The XvRF17 protein was predicted by in silico analyses to belong to the family of metallothioneins, a family of metal-binding proteins. Anaylis of the XvRG6 mRNA transcript showed that the gene was not dehydration stress inducible, but was induced by ethylene, endogenous ABA, SA and JA application. In contrast, the XvRF17 mRNA transcript was shown by RT-PCR to be induced by dehydration stress, endogenous ABA, SA and JA application but not by ethylene treatment. Interestingly, western blot analyses revealed that XvRG6 protein levels increased only during dehydration and not during any other imposed stress. This study has shown that both XvRG6 and XvRF17 and responsive to stress. Since there are no known orthologues of these genes, their respective roles in X. viscosa remains speculative. 2014-07-30T17:37:34Z 2014-07-30T17:37:34Z 2007 Master Thesis Masters MSc http://hdl.handle.net/11427/4261 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Felix, Mba Medie Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa |
| thesis_degree_str | Master's |
| title | Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa |
| title_full | Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa |
| title_fullStr | Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa |
| title_full_unstemmed | Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa |
| title_short | Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa |
| title_sort | molecular characterisation of xvrg6 and xvrf17 genes isolated from the resurrection plant xeropyta viscosa |
| url | http://hdl.handle.net/11427/4261 |
| work_keys_str_mv | AT felixmbamedie molecularcharacterisationofxvrg6andxvrf17genesisolatedfromtheresurrectionplantxeropytaviscosa |