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The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613218773401600 |
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| access_status_str | Open Access |
| author | Hedley, Paula |
| author2 | O'Ryan, Colleen |
| author_browse | Hedley, Paula O'Ryan, Colleen |
| author_facet | O'Ryan, Colleen Hedley, Paula |
| author_sort | Hedley, Paula |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4271 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:39.476Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4271 The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations Hedley, Paula O'Ryan, Colleen Cell Biology Includes bibliographical references. This thesis reports the development of Single-Strand Conformation Polymorphism (SSCP) technique to assess sequence level variation within the Major Histocompatibility Complex (MHC) DRB1 gene in four South African buffalo populations. MHC gene products are involved in the immune response, and so variation within these genes provides information on the immunological fitness of the population under study. The aims of this study were: (i) to develop the SSCP technique; (ii) to investigate the level of genetic variation at the peptide binding region (PBR) of the DRB1 gene in four South African buffalo populations. (iii) This data was then compared to data generated previously in a study on the same populations using microsatellite DNA, (iv) the statistical comparisons were used to assess the appropriateness of SSCP data for population genetic analysis. Levels of heterozygosity, allelic diversity and population differentiation were quantified using MHC DRB1 gene. The amplified region (Exon 2 of the DRB1 gene) showed high levels of variability, with 77 alleles found in the 84 individuals examined using SSCP analyses. 2014-07-30T17:38:26Z 2014-07-30T17:38:26Z 2003 Master Thesis Masters MSc http://hdl.handle.net/11427/4271 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Hedley, Paula The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations |
| thesis_degree_str | Master's |
| title | The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations |
| title_full | The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations |
| title_fullStr | The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations |
| title_full_unstemmed | The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations |
| title_short | The development of the single-strand conformation polymorphism (SSCP) technique to assess sequence level variation within the major histocompatibility complex (MHC) DRB1 gene in four South African buffalo (Syncerus caffer) populations |
| title_sort | development of the single strand conformation polymorphism sscp technique to assess sequence level variation within the major histocompatibility complex mhc drb1 gene in four south african buffalo syncerus caffer populations |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4271 |
| work_keys_str_mv | AT hedleypaula thedevelopmentofthesinglestrandconformationpolymorphismsscptechniquetoassesssequencelevelvariationwithinthemajorhistocompatibilitycomplexmhcdrb1geneinfoursouthafricanbuffalosynceruscafferpopulations AT hedleypaula developmentofthesinglestrandconformationpolymorphismsscptechniquetoassesssequencelevelvariationwithinthemajorhistocompatibilitycomplexmhcdrb1geneinfoursouthafricanbuffalosynceruscafferpopulations |