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Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse
Includes abstract.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613327706816512 |
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| access_status_str | Open Access |
| author | Nembaware, Victoria Precious |
| author2 | Seoighe, Cathal |
| author_browse | Nembaware, Victoria Precious Seoighe, Cathal |
| author_facet | Seoighe, Cathal Nembaware, Victoria Precious |
| author_sort | Nembaware, Victoria Precious |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4310 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:23.309Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4310 Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse Nembaware, Victoria Precious Seoighe, Cathal Cell Biology Includes abstract. Includes bibliographical references (leaves 125-145). This dissertation aims to examine allele-specific splicing in human and mouse using publicly available datasets. Such datasets, which have been generated from multiple tissue sources and from individuals of diverse backgrounds, are rich and cheap reservoirs of transcript isoforms resulting from alternative splicing as well as isoforms resulting from mutations or polymorphisms (allele-specific isoforms). Published tools were used to analyse microarray and genomic data. However, for the assessment of allele-specific splicing using publicly available high-throughput transcript sequences, we present two novel methods: a heuristic method for quantifying the prevalence of allele-specific splicing and a more sophisticated maximum likelihood method for the detection of individual examples of allele-specific splicing. These methods make use of transcripts that can be mapped to both polymorphisms and computationally predicted mRNA isoforms. Inference of polymorphic alleles from transcripts is laborious hence a pre-computed database was created for the human data and made publicly available for use by the wider research community. 2014-07-30T17:40:29Z 2014-07-30T17:40:29Z 2008 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/4310 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Nembaware, Victoria Precious Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse |
| thesis_degree_str | Doctoral |
| title | Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse |
| title_full | Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse |
| title_fullStr | Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse |
| title_full_unstemmed | Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse |
| title_short | Genome-wide survey and analysis of allele-specific mRNA splicing in human and mouse |
| title_sort | genome wide survey and analysis of allele specific mrna splicing in human and mouse |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4310 |
| work_keys_str_mv | AT nembawarevictoriaprecious genomewidesurveyandanalysisofallelespecificmrnasplicinginhumanandmouse |