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Expression of HPV-16 L2 in plants
Includes bibliographical references (leaves 77-84).
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
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| _version_ | 1867613325556187136 |
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| access_status_str | Open Access |
| author | Pereira, Roman |
| author2 | Rybicki, Ed |
| author_browse | Pereira, Roman Rybicki, Ed |
| author_facet | Rybicki, Ed Pereira, Roman |
| author_sort | Pereira, Roman |
| collection | Thesis |
| description | Includes bibliographical references (leaves 77-84). |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4317 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:20.437Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4317 Expression of HPV-16 L2 in plants Pereira, Roman Rybicki, Ed Becker, Inga Maclean, James Cell Biology Includes bibliographical references (leaves 77-84). This study demonstrates high level expression of L2, 25 mg.kg⁻¹of leaf material, is achievable. Interestingly, expression was best when coded for by a mammalian codon-optimized form of the L2 gene as opposed to the wildtype or plant codon-optimized (plantized) genes. Moreover, real time PCR revealed limited levels of transcript when coded for by the plantized gene in comparison to the other genes. A set of vectors which target the protein to the cytoplasm, the endoplasmic reticulum (ER), chloroplast or apoplastic space were Expression of HPV -16 L2 in Plants used and it was found that targeting of the protein had no effect on its expression levels. 2014-07-30T17:41:28Z 2014-07-30T17:41:28Z 2008 Master Thesis Masters MSc http://hdl.handle.net/11427/4317 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Pereira, Roman Expression of HPV-16 L2 in plants |
| thesis_degree_str | Master's |
| title | Expression of HPV-16 L2 in plants |
| title_full | Expression of HPV-16 L2 in plants |
| title_fullStr | Expression of HPV-16 L2 in plants |
| title_full_unstemmed | Expression of HPV-16 L2 in plants |
| title_short | Expression of HPV-16 L2 in plants |
| title_sort | expression of hpv 16 l2 in plants |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4317 |
| work_keys_str_mv | AT pereiraroman expressionofhpv16l2inplants |