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The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
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| _version_ | 1867613203754647552 |
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| access_status_str | Open Access |
| author | Somers, Sachin J |
| author2 | Patterton, Hugh |
| author_browse | Patterton, Hugh Somers, Sachin J |
| author_facet | Patterton, Hugh Somers, Sachin J |
| author_sort | Somers, Sachin J |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4335 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:24.523Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4335 The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase Somers, Sachin J Patterton, Hugh Cell Biology Includes bibliographical references. Our group has recently found that the linker histone Hh01 p of Saccharomyces cerevisiae exhibited a significant increase in binding to chromatin during stationary phase. Because of the role of H1 in gene expression and chromatin compaction, it is essential to understand the mechanism behind this change in binding behaviour for a complete mechanistic description of gene regulation. We postulated that the phosphorylation of serine or threonine residues decrease the affinity of H1 for DNA, resulting in the dissociation of H1 from chromatin in exponential phase. We investigated this possible change in the phosphorylation state of Hh01 p in yeast cells in exponential phase and in stationary phase by immunoprecipitation of Hh01 p, followed by western analysis using antiphosphoserine and anti-phosphothreonine antibodies. 2014-07-30T17:41:58Z 2014-07-30T17:41:58Z 2005 Master Thesis Masters MSc http://hdl.handle.net/11427/4335 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Somers, Sachin J The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase |
| thesis_degree_str | Master's |
| title | The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase |
| title_full | The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase |
| title_fullStr | The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase |
| title_full_unstemmed | The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase |
| title_short | The phosphorylation state of Saccharomyces cerevisiae linker histone Hho 1p during entry and exit of stationary phase |
| title_sort | phosphorylation state of saccharomyces cerevisiae linker histone hho 1p during entry and exit of stationary phase |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4335 |
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