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Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco
Includes abstract.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613316250075136 |
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| access_status_str | Open Access |
| author | Stark, Hester Catharina (Therese) |
| author2 | Rybicki, Ed |
| author_browse | Rybicki, Ed Stark, Hester Catharina (Therese) |
| author_facet | Rybicki, Ed Stark, Hester Catharina (Therese) |
| author_sort | Stark, Hester Catharina (Therese) |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4336 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:10.861Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4336 Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco Stark, Hester Catharina (Therese) Rybicki, Ed Chikwamba, Rachel Cell Biology Includes abstract. Includes bibliographical references. Plants are emerging as cost friendly alternative production systems for a variety of pharmaceuticals. Numerous therapeutic proteins have been produced in plant systems (Giddings et al., 2000; Ma et al., 2003). Protein based microbicides,-namely, neutralising antibodies and peptide lectins- lend themselves to production in plants (De Muynck et al., 2010; Matoba et al., 2010; Sexton et al. 2006; O’Keefe et al., 2009). One of these lectins, namely Griffithsin (GRFT) was isolated from the blue green algae Griffithsia and is being developed as a leading anti-HIV microbicide peptide (Mori et al., 2005). As literature indicates, the optimal production of any protein is an empirical experimentation with different host systems, vector systems, codon optimisations and subcellular targeting. (Maclean et al., 2007; Yang et al., 2005). The latter sometimes results in unexpected locations which might reflect on an inherent property of the protein itself or specifically be associated with the plant organ involved (Chikwamba et al., 2003). This again can influence protein yield- and activity, and impact downstream purification. In this study we aimed to compare expression levels using both Zea mays (maize) and Nicotiana benthamiana (tobacco) with relevant vector technologies. We expressed GRFT in maize using an endosperm specific maize expression vector with and without a signal peptide. In tobacco we utilised both the pTRA binary vector and magnICON deconstructed viral vector system to express GRFT with different subcellular targeting signals. 2014-07-30T17:41:59Z 2014-07-30T17:41:59Z 2013 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/4336 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Stark, Hester Catharina (Therese) Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco |
| thesis_degree_str | Doctoral |
| title | Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco |
| title_full | Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco |
| title_fullStr | Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco |
| title_full_unstemmed | Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco |
| title_short | Targeted expression of the anti-HIV microbicide lectin griffithsin in maize and tobacco |
| title_sort | targeted expression of the anti hiv microbicide lectin griffithsin in maize and tobacco |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4336 |
| work_keys_str_mv | AT starkhestercatharinatherese targetedexpressionoftheantihivmicrobicidelectingriffithsininmaizeandtobacco |