Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis
Bibliography: leaves 24-29.
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613340015001600 |
|---|---|
| access_status_str | Open Access |
| author | Tawouokam, Jean Bernard |
| author_browse | Tawouokam, Jean Bernard |
| author_facet | Tawouokam, Jean Bernard |
| author_sort | Tawouokam, Jean Bernard |
| collection | Thesis |
| description | Bibliography: leaves 24-29. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4338 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:33.896Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4338 Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis Tawouokam, Jean Bernard Molecular and cell biology Bibliography: leaves 24-29. Low temperature is one of the environmental factors that cause substantial crop losses in the world. Recent advances in the study of plants native from temperate regions, have established that cold acclimation is regulated at the gene expression leveL To study the function ofaXerophyta viscosa cold responsive protein that accumulates in plant cells under various environmental stresses, we have cloned the Xerophyta viscosa cold-responsive cDNA gene designed Xvcor, into a less complex host, Saccharomyces cerevisiae with the purpose of carrying out in vivo functional analysis. Sequence analysis showed that Xvcor encodes a 264 amino acid residue protein (Garwe et al 2002). The Hydropathy plot indicated that the XVCOR protein is highly hydrophobic and contains 6 transmembrane domains (Garwe et al 2002). In order to achieve high-level expression of Xvcor, the gene was placed under the control of a strong promoter (Phosphoglycerate kinase). Reverse-transcription PCR amplification revealed that the Xvcor transcript accumulated in yeast cells. However, SDS-PAGE analysis could not detect the predicted 29.6 kDa recombinant protein, suggesting that the translation might be hampered. Tests designed to measure the enhancement of stress tolerance between the recombinant and the control showed similar growth performance, confirming the absence of recombinant protein. A computer search for codon usage showed that the codon usage bias in Xvcor was low compared with that of highly expressed genes of S. cerevisiae. Together, these results suggest that the codon usage in Xvcor could influence its expression in yeast. 2014-07-30T17:42:04Z 2014-07-30T17:42:04Z 2002 Master Thesis Masters MSc http://hdl.handle.net/11427/4338 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Molecular and cell biology Tawouokam, Jean Bernard Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis |
| thesis_degree_str | Master's |
| title | Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis |
| title_full | Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis |
| title_fullStr | Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis |
| title_full_unstemmed | Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis |
| title_short | Attempt to express the Xerophyta viscose stress-responsive gene, Xvcor, in yeast with view to functional analysis |
| title_sort | attempt to express the xerophyta viscose stress responsive gene xvcor in yeast with view to functional analysis |
| topic | Molecular and cell biology |
| url | http://hdl.handle.net/11427/4338 |
| work_keys_str_mv | AT tawouokamjeanbernard attempttoexpressthexerophytaviscosestressresponsivegenexvcorinyeastwithviewtofunctionalanalysis |