Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery
Luciferins are a class of small, light-emitting molecules that produce visible light through an oxidation reaction catalysed by luciferase enzymes. This bioluminescent system, when combined with CCD camera technologies, has enabled groundbreaking advances in measuring mammalian gene expression, prot...
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English English |
| Published: |
Department of Chemistry
2026
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1869483667629277184 |
|---|---|
| access_status_str | Open Access |
| author | Fredericks, Maryam |
| author2 | Jardine, Mogamat |
| author_browse | Fredericks, Maryam Jardine, Mogamat |
| author_facet | Jardine, Mogamat Fredericks, Maryam |
| author_sort | Fredericks, Maryam |
| collection | Thesis |
| description | Luciferins are a class of small, light-emitting molecules that produce visible light through an oxidation reaction catalysed by luciferase enzymes. This bioluminescent system, when combined with CCD camera technologies, has enabled groundbreaking advances in measuring mammalian gene expression, protein–protein interactions, biochemical labelling, and small molecule dynamics. Among the luciferins, D-luciferin—originally isolated from the American firefly Photinus pyralis—is the most extensively studied. Synthetic analogues of D-luciferin have become increasingly important due to their incorporation into a wide range of commercially available assay kits, where they serve as sensitive reporters in both in vitro and in vivo applications. This thesis focuses on the development of improved synthetic methods for D-thioluciferin, C6-substituted analogues, and aza-derivatives of D-luciferin, the latter enabling the design of novel caged bioluminescent probes for advanced molecular imaging. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/43385 |
| institution | University of Cape Town (South Africa) |
| language | English eng |
| last_indexed | 2026-07-01T04:02:38.759Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2026 |
| publishDateRange | 2026 |
| publishDateSort | 2026 |
| publisher | Department of Chemistry |
| publisherStr | Department of Chemistry |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/43385 Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery Fredericks, Maryam Jardine, Mogamat bioluminescent probes drug Luciferins are a class of small, light-emitting molecules that produce visible light through an oxidation reaction catalysed by luciferase enzymes. This bioluminescent system, when combined with CCD camera technologies, has enabled groundbreaking advances in measuring mammalian gene expression, protein–protein interactions, biochemical labelling, and small molecule dynamics. Among the luciferins, D-luciferin—originally isolated from the American firefly Photinus pyralis—is the most extensively studied. Synthetic analogues of D-luciferin have become increasingly important due to their incorporation into a wide range of commercially available assay kits, where they serve as sensitive reporters in both in vitro and in vivo applications. This thesis focuses on the development of improved synthetic methods for D-thioluciferin, C6-substituted analogues, and aza-derivatives of D-luciferin, the latter enabling the design of novel caged bioluminescent probes for advanced molecular imaging. 2026-06-25T10:11:54Z 2026-06-25T10:11:54Z 2026 2026-06-25T09:58:07Z Thesis / Dissertation Masters MSc http://hdl.handle.net/11427/43385 en eng application/pdf Department of Chemistry Faculty of Science University of Cape Town |
| spellingShingle | bioluminescent probes drug Fredericks, Maryam Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| thesis_degree_str | Master's |
| title | Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| title_full | Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| title_fullStr | Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| title_full_unstemmed | Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| title_short | Synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| title_sort | synthesis and evaluation of bioluminescent probes as tools for anticancer drug discovery |
| topic | bioluminescent probes drug |
| url | http://hdl.handle.net/11427/43385 |
| work_keys_str_mv | AT fredericksmaryam synthesisandevaluationofbioluminescentprobesastoolsforanticancerdrugdiscovery |