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Experimental investigations of mastrevirus molecular biology and evolution
Includes abstract.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
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| _version_ | 1867613230499627008 |
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| access_status_str | Open Access |
| author | Van der Walt, Eric |
| author2 | Rybicki, Ed |
| author_browse | Rybicki, Ed Van der Walt, Eric |
| author_facet | Rybicki, Ed Van der Walt, Eric |
| author_sort | Van der Walt, Eric |
| collection | Thesis |
| description | Includes abstract. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/4346 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:32:50.328Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/4346 Experimental investigations of mastrevirus molecular biology and evolution Van der Walt, Eric Rybicki, Ed Martin, D P Cell Biology Includes abstract. Includes bibliographical references (leaves 138-161). This dissertation describes three major sets of experiments, all of which involved the construction and use of various reciprocal chimaeric MSV constructs. First, chimaeric viruses were used in genetic complementation-type experiments to investigate the biological significance of interactions between the two virion-sense open reading frames (ORFs) of MSV, their products, and the rest of the genome. Six chimaeric MSV constructs were made by reciprocally exchanging the ORFs encoding movement protein (MP) and coat protein (CP) individually, and in pairs, between MSV-Kom and MSV-Set, which share just 78% overall nucleotide identity. Analysis of symptomatology and infection efficiency of chimaeras and wild-type parental viruses revealed evidence of functionally relevant specific interactions between MSV MP and CP. 2014-07-30T17:42:15Z 2014-07-30T17:42:15Z 2008 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/4346 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Van der Walt, Eric Experimental investigations of mastrevirus molecular biology and evolution |
| thesis_degree_str | Doctoral |
| title | Experimental investigations of mastrevirus molecular biology and evolution |
| title_full | Experimental investigations of mastrevirus molecular biology and evolution |
| title_fullStr | Experimental investigations of mastrevirus molecular biology and evolution |
| title_full_unstemmed | Experimental investigations of mastrevirus molecular biology and evolution |
| title_short | Experimental investigations of mastrevirus molecular biology and evolution |
| title_sort | experimental investigations of mastrevirus molecular biology and evolution |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/4346 |
| work_keys_str_mv | AT vanderwalteric experimentalinvestigationsofmastrevirusmolecularbiologyandevolution |