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The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development
Includes bibliographical references.
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
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| _version_ | 1867614118202048512 |
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| access_status_str | Open Access |
| author | Shoko, Aubrey Themba |
| author_browse | Shoko, Aubrey Themba |
| author_facet | Shoko, Aubrey Themba |
| author_sort | Shoko, Aubrey Themba |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9028 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:46:57.487Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9028 The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development Shoko, Aubrey Themba Cell Biology Includes bibliographical references. Due to its unique capacity to regenerate continously, the olfactory neuroepithelium serves as an excellent model system for investigating the molecular and cellular mechanisms involved in neurogenesis. The OP27 cell line (generated by infecting embryonic mouse olfactory placodes with a retrovirus carrying the temperature-sensitive alleles of the SV 40 large T antigen) was used as an in vitro system to test the effects of fibroblast growth factor-2 (FGF-2) in directing olfactory neurogenesis. The OP27 cells proliferate under the control of the retrovirus at the permissive temperature (33°C). When shifted to the non-permissive temperature (39°C) the SV40 large T antigen is inactivated and the cells stop dividing, thereby allowing one to study the effects of growth factors on these cells. Although FGF-2 also plays an important role in regulating the proliferation of neural progenitors, the main focus in this study was its effect on neuronal differentiation. 2014-11-03T08:14:22Z 2014-11-03T08:14:22Z 2004 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/9028 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Cell Biology Shoko, Aubrey Themba The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development |
| thesis_degree_str | Doctoral |
| title | The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development |
| title_full | The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development |
| title_fullStr | The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development |
| title_full_unstemmed | The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development |
| title_short | The OP27 cell line as a model system to study the effects of FGF-2 in olfactory neuronal development |
| title_sort | op27 cell line as a model system to study the effects of fgf 2 in olfactory neuronal development |
| topic | Cell Biology |
| url | http://hdl.handle.net/11427/9028 |
| work_keys_str_mv | AT shokoaubreythemba theop27celllineasamodelsystemtostudytheeffectsoffgf2inolfactoryneuronaldevelopment AT shokoaubreythemba op27celllineasamodelsystemtostudytheeffectsoffgf2inolfactoryneuronaldevelopment |