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Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression
Includes bibliographical references.
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613171178536960 |
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| access_status_str | Open Access |
| author | Wolf, Michael |
| author2 | Ingle, Robert |
| author_browse | Ingle, Robert Wolf, Michael |
| author_facet | Ingle, Robert Wolf, Michael |
| author_sort | Wolf, Michael |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9109 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:53.390Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9109 Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression Wolf, Michael Ingle, Robert Bishop, Jacqueline Muasya, Muthama Includes bibliographical references. Heavy metal (HM) accumulator plants possess the ability to actively hyperaccumulate and detoxify exceptionally high concentrations of metals in their aboveground tissues, without exhibiting any apparent signs of toxicity. Despite nickel (Ni) hyperaccumulator plants representing the largest percentage of known metal accumulator taxa (over 75%), the underlying genetic and molecular basis of Ni accumulation remains unclear. A prominent difficulty in understanding Ni hyperaccumulation has been the severe lack of intraspecific variation in the trait. Hence, the study of a single species exhibiting a significant degree of variation is highly desirable. as it avoids the use of inter-species comparative studies mostly utilized to date. The Ni hyperaccumulator Senecio coronatus (Asteraceae) has been reported to contain a significant degree of phenotypic plasticity with respect to the amount accumulated and subsequent cellular distribution of Ni. This apparent intraspecific variation means that S. coronatus may represent a useful system in which to study Ni hyperaccumulation. No population genetics study has been carried out to date on this species, and the evolutionary relationships between hyper and non- accumulator populations were unknown. Here, results are presented from a genetic analysis of 15 naturally occurring S. coronatus populations. Analysis of molecular variance (AMOVA) and phylogenetic analysis (based on non-coding nuclear and plastid markers) suggest that Ni accumulation may have evolved twice within S. coronatus, as hyperaccumulator plants from site Kaapsehoop, cluster with non-accumulating serpentine populations and demonstrate distinct genetic differentiation from other accumulator populations. Four populations were selected for a preliminary comparative shoot proteome analysis by means of two-dimensional SDS-polyacrylamide gel electrophoresis (2D SDS-PAGE) to identify proteins potentially involved in Ni hyperaccumulation. This analysis identified nine chloroplastic proteins involved in plant energy production and metabolism as overexpressed in hyperaccumulator plants from Agnus Mine and Kaapsehoop, compared to hypertolerant non-accumulator and non-serpentine plants from Galaxy Mine and Pullen Farm, respectively. However, no difference in photosynthetic efficiency, as determined by chlorophyll fluorescence measurements, was detected between these populations. 2014-11-05T03:46:47Z 2014-11-05T03:46:47Z 2013 Master Thesis Masters MSc http://hdl.handle.net/11427/9109 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Wolf, Michael Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression |
| thesis_degree_str | Master's |
| title | Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression |
| title_full | Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression |
| title_fullStr | Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression |
| title_full_unstemmed | Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression |
| title_short | Characterization of the intraspecific variation within the nickel (Ni) hyperaccumulator species Senecio coronatus (Asteraceae): a preliminary analysis of genetic population structure and shoot proteome expression |
| title_sort | characterization of the intraspecific variation within the nickel ni hyperaccumulator species senecio coronatus asteraceae a preliminary analysis of genetic population structure and shoot proteome expression |
| url | http://hdl.handle.net/11427/9109 |
| work_keys_str_mv | AT wolfmichael characterizationoftheintraspecificvariationwithinthenickelnihyperaccumulatorspeciesseneciocoronatusasteraceaeapreliminaryanalysisofgeneticpopulationstructureandshootproteomeexpression |