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Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining
Includes bibliographical references.
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| Main Author: | |
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| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
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| _version_ | 1867613251202711552 |
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| access_status_str | Open Access |
| author | Moonsamy, Karusha |
| author2 | Coyne, Vernon |
| author_browse | Coyne, Vernon Moonsamy, Karusha |
| author_facet | Coyne, Vernon Moonsamy, Karusha |
| author_sort | Moonsamy, Karusha |
| collection | Thesis |
| description | Includes bibliographical references. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9110 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:33:10.259Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9110 Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining Moonsamy, Karusha Coyne, Vernon Includes bibliographical references. Bioluminescence imaging (BLI) is a powerful technique that enables real time monitoring of various processes such as gene regulation, host-pathogen interaction and distribution of bacterial cells in vivo. Most of these processes however have only been monitored in murine models or mammalian tissue culture systems. In order to determine whether BLI could be used to monitor bacterial-host interactions in an invertebrate system, the probiotic bacterium Vibrio midae SY9 was genetically labelled with a bioluminescent plasmid (pKluxCat) containing the lux operon from Photorhabdus luminescens. The plasmid was modified by the addition of the Tn9 chloramphenicol acetyltransferase (cat) gene to enable selection of Vibrio transconjugants. The bacterial cells were labelled using a conjugal mating technique and the resulting transconjugant cells were highly bioluminescent with a minimum of 5.264 x104 colony forming units detectable in liquid culture. The bioluminescent cells emitted light stably and consistently without compromising the growth of the strain, and bioluminescence emission was at its highest during the logarithmic growth phase. A strong linear correlation between bioluminescence emitted and bacterial numbers (r2=0.99) was found, indicating that the amount of bacteria present in vivo could be accurately quantitated using bioluminescence. Administration of the bioluminescent bacterial cells by oral gavage to juvenile Haliotis midae abalone allowed non-invasive visualisation of bioluminescence within abalone for up to 10 hours post-gavage. This study therefore demonstrates the potential for using bioluminescence imaging to investigate the interaction of bacteria with an invertebrate host. 2014-11-05T03:46:55Z 2014-11-05T03:46:55Z 2014 Master Thesis Masters MSc http://hdl.handle.net/11427/9110 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Moonsamy, Karusha Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining |
| thesis_degree_str | Master's |
| title | Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining |
| title_full | Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining |
| title_fullStr | Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining |
| title_full_unstemmed | Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining |
| title_short | Visualisation of bioluminescent Vibrio midae SY9 K811 in juvenile Haliotis midae abalone using non-invasive bioluminescence imagining |
| title_sort | visualisation of bioluminescent vibrio midae sy9 k811 in juvenile haliotis midae abalone using non invasive bioluminescence imagining |
| url | http://hdl.handle.net/11427/9110 |
| work_keys_str_mv | AT moonsamykarusha visualisationofbioluminescentvibriomidaesy9k811injuvenilehaliotismidaeabaloneusingnoninvasivebioluminescenceimagining |