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Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor
Bibliography: leaves 116-120.
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| Main Author: | |
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867613170711920640 |
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| access_status_str | Open Access |
| author | Ma, Pei-yin |
| author_browse | Ma, Pei-yin |
| author_facet | Ma, Pei-yin |
| author_sort | Ma, Pei-yin |
| collection | Thesis |
| description | Bibliography: leaves 116-120. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9241 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:31:53.390Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9241 Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor Ma, Pei-yin Biochemistry Bibliography: leaves 116-120. Wound-inducible proteinase inhibitors in plants elicit a defence mechanism by inactivating the proteinases of insects. This triggers a feedback mechanism causing overproduction of digestive enzymes together with a decrease in appetite, leading to starvation. System in, a polypeptide proteinase inhibitor-inducing factor, when applied to cut stems of young tomato plants induces the accumulation of inhibitors in a manner similar to the normal wounding response. We designed and synthesised the minus strand oligonucleotide template complementary to the system in DNA sequence using Escherichia coli codon preferences. The double stranded fragment encoding the 18 amino acid residue systemin was cloned into pUCJ 8 for amplification and subcloning into pMAL-pk for expression as a maltose binding-fusion protein. The recombinant systemin was released by enterokinase and isolated by HPLC. After further purification, the physical characteristics including amino acid composition, peptide sequence and molecular weight of r-systemin were determined. When the recombinant peptide was applied to young tomato plants, it induced the accumulation of proteinase inhibitor I messenger RNA. 2014-11-05T17:31:18Z 2014-11-05T17:31:18Z 1996 Master Thesis Masters MSc http://hdl.handle.net/11427/9241 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Biochemistry Ma, Pei-yin Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor |
| thesis_degree_str | Master's |
| title | Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor |
| title_full | Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor |
| title_fullStr | Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor |
| title_full_unstemmed | Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor |
| title_short | Chemical synthesis, cloning and expression of a gene encoding systemin, a proteinase inhibitor-inducing factor |
| title_sort | chemical synthesis cloning and expression of a gene encoding systemin a proteinase inhibitor inducing factor |
| topic | Biochemistry |
| url | http://hdl.handle.net/11427/9241 |
| work_keys_str_mv | AT mapeiyin chemicalsynthesiscloningandexpressionofageneencodingsysteminaproteinaseinhibitorinducingfactor |