Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262
Bibliography: leaves [168]-207.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613304465129472 |
|---|---|
| access_status_str | Open Access |
| author | Collett, Helen Jeanne |
| author2 | Reid, Sharon J |
| author_browse | Collett, Helen Jeanne Reid, Sharon J |
| author_facet | Reid, Sharon J Collett, Helen Jeanne |
| author_sort | Collett, Helen Jeanne |
| collection | Thesis |
| description | Bibliography: leaves [168]-207. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9243 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:34:00.978Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9243 Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 Collett, Helen Jeanne Reid, Sharon J Microbiology Bibliography: leaves [168]-207. Metronidazole is a pro-drug which must be reduced to elicit a bactericidal effect. In the clostridia, some of the electron transport proteins that provide the source of electrons for the reductive activation of metronidazole play a key role in electron distribution, which in turn regulates the direction of carbon flow in the cell. The aim of this research project was to isolate electron transport gene(s) from the solvent-producing Clostridium acetobutylicum strain P262, using transposon-induced metronidazole resistance as a selection system. In the process, the feasibility of transposon mutagenesis in this strain, which lacks conventional systems for DNA delivery, was assessed, and the nature of metronidazole susceptibility in the C. acetobutylicum wild type was investigated. The metronidazole resistant transconjugant of interest, referred to as mutant 3R, was shown to harbour a single insertion of the Tn925: :Tn917 transposon cointegrate within a structural gene, designated sum (susceptibility to metronidazole). 2014-11-05T17:31:24Z 2014-11-05T17:31:24Z 1996 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/9243 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Collett, Helen Jeanne Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 |
| thesis_degree_str | Doctoral |
| title | Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 |
| title_full | Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 |
| title_fullStr | Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 |
| title_full_unstemmed | Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 |
| title_short | Characterization of a transposon-induced pleiotropic metronidazole resistant mutant of Clostridium acetobutylicum P262 |
| title_sort | characterization of a transposon induced pleiotropic metronidazole resistant mutant of clostridium acetobutylicum p262 |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/9243 |
| work_keys_str_mv | AT colletthelenjeanne characterizationofatransposoninducedpleiotropicmetronidazoleresistantmutantofclostridiumacetobutylicump262 |