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Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities
Bibliography: leaves 128-146.
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| Format: | Thesis |
| Language: | English |
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Department of Molecular and Cell Biology
2014
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| _version_ | 1867614238401363968 |
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| access_status_str | Open Access |
| author | Haworth, Caroline Joanne |
| author2 | Thomson, Jennifer Ann |
| author_browse | Haworth, Caroline Joanne Thomson, Jennifer Ann |
| author_facet | Thomson, Jennifer Ann Haworth, Caroline Joanne |
| author_sort | Haworth, Caroline Joanne |
| collection | Thesis |
| description | Bibliography: leaves 128-146. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9485 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:48:52.118Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9485 Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities Haworth, Caroline Joanne Thomson, Jennifer Ann Maeder, Dennis Biochemistry Bibliography: leaves 128-146. Cysteine proteinase inhibitors have shown potential as biocontrol agents for the protection of plants against insect and pathogen attack. With the advent of protein and genetic engineering such inhibitors can now be modified in order to improve their effectiveness. Because cystatins have already been isolated from plants. they provide a good starting point for developing modifications which may improve their function as biocontrol agents. The purpose of this project, therefore, was to design a potentially improved analogue of the rice cysteine proteinase inhibitor, oryzacystatin I, through molecular modelling studies. The gene sequence for this modified protein was then synthesised and expressed for kinetic analysis and insect trial assays. A prediction of the oryzacystatin I (OC I) tertiary structure was made using Biograf software on an Evans and Sutherland workstation. This structure was based on the known structures of stefin B and chicken cystatin ho se co-ordinates are published in the Brookhaven data files. Chicken cystatin is one of the most potent inhibitors of papain in the cystatin superfamily. This is believed to be due, in part, to an increased binding of the cystatin to papain through its amino-terminal region with the residues Leu7 to Gly9 playing a particularly important role. 2014-11-10T08:59:53Z 2014-11-10T08:59:53Z 1997 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/9485 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Biochemistry Haworth, Caroline Joanne Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| thesis_degree_str | Doctoral |
| title | Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| title_full | Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| title_fullStr | Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| title_full_unstemmed | Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| title_short | Cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| title_sort | cloning and expression of a modified oryzacystatin inhibitor gene and an investigation of its inhibitory capabilities |
| topic | Biochemistry |
| url | http://hdl.handle.net/11427/9485 |
| work_keys_str_mv | AT haworthcarolinejoanne cloningandexpressionofamodifiedoryzacystatininhibitorgeneandaninvestigationofitsinhibitorycapabilities |