Full Text Available
Note: Clicking the button above will open the full text document at the original institutional repository in a new window.
Studies on the regulation of extracellular collagenase production by vibrio alginolyticus
Bibliography: leaves 117-133.
Saved in:
| Main Author: | |
|---|---|
| Other Authors: | |
| Format: | Thesis |
| Language: | English |
| Published: |
Department of Molecular and Cell Biology
2014
|
| Subjects: | |
| Tags: |
No Tags, Be the first to tag this record!
|
| _version_ | 1867613601862254592 |
|---|---|
| access_status_str | Open Access |
| author | Reid, Graham Charlton |
| author2 | Woods, David R |
| author_browse | Reid, Graham Charlton Woods, David R |
| author_facet | Woods, David R Reid, Graham Charlton |
| author_sort | Reid, Graham Charlton |
| collection | Thesis |
| description | Bibliography: leaves 117-133. |
| format | Thesis |
| id | oai:open.uct.ac.za:11427/9978 |
| institution | University of Cape Town (South Africa) |
| language | eng |
| last_indexed | 2026-06-10T12:38:45.067Z |
| license_str | Not specified — see source repository |
| provenance_str_mv | Harvested via OAI-PMH from UCTD — University of Cape Town Open Access Repository |
| publishDate | 2014 |
| publishDateRange | 2014 |
| publishDateSort | 2014 |
| publisher | Department of Molecular and Cell Biology |
| publisherStr | Department of Molecular and Cell Biology |
| record_format | dspace |
| source_str | UCTD — University of Cape Town Open Access Repository |
| spelling | oai:open.uct.ac.za:11427/9978 Studies on the regulation of extracellular collagenase production by vibrio alginolyticus Reid, Graham Charlton Woods, David R Robb, Frank T Microbiology Bibliography: leaves 117-133. Vibrio alginolyticus synthesized extracellular collagenase in a highly aerated peptone medium at the late-exponential and early-stationary phases of growth. Collagenase synthesis was subject to end-product repression and was repressed by various amino acids and ammonium ions. Glutamine caused severe repression of collagenase production. Collagenase synthesis was sensitive to catabolite repression by glucose and a number of carbon sources. Cyclic AMP, dibutyryl cyclic AMP and cyclic GMP did not relieve catabolite repression. Glucose and 2-deoxyglucose caused a severe transient repression. No intracellular preformed collagenase was detected and collagenase production ceased when induced cells were washed and resuspended in buffer.Trypsin and a-chymotrypsin had no effect on collagenase production by cells or sphaeroplasts. The inducers of collagenase production in peptone were shown to have abroad molecular weight range between 1, 000 and 60,000. The peptone inducers supported slow growth of V. alginolyticus when supplied as the sole nitrogen source in minimal medium. Digestion of the peptone inducers with purified V. alginolyticus collagenase resulted in a decrease in their inducing ability,whereas digestion with trypsin or a-chymotrypsin did not. Peptone acted as an inhibitor of collagenase. 2014-12-13T06:22:46Z 2014-12-13T06:22:46Z 1981 Doctoral Thesis Doctoral PhD http://hdl.handle.net/11427/9978 eng application/pdf Department of Molecular and Cell Biology Faculty of Science University of Cape Town |
| spellingShingle | Microbiology Reid, Graham Charlton Studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| thesis_degree_str | Doctoral |
| title | Studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| title_full | Studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| title_fullStr | Studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| title_full_unstemmed | Studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| title_short | Studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| title_sort | studies on the regulation of extracellular collagenase production by vibrio alginolyticus |
| topic | Microbiology |
| url | http://hdl.handle.net/11427/9978 |
| work_keys_str_mv | AT reidgrahamcharlton studiesontheregulationofextracellularcollagenaseproductionbyvibrioalginolyticus |