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Genetic variants of Plasmodium falciparum infective Anopheles gambiae s.l. at a rural community in southwest Nigeria
During studies on the epidemiology of malaria at a rural community, Igbo-Ora, Southwest Nigeria, genotyping of Plasmodium falciparum extracted from infective Anopheles gambiae s.l. was undertaken. Circumsporozoite (CSP) ELISA was used on crushes from head-thorax for DNA extraction and PCR amplificat...
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2009
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| LEADER | 00000njm a2000000a 4500 | ||
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| 001 | oai:repository.ui.edu.ng:123456789/1060 | ||
| 042 | |a dc | ||
| 720 | |a Noutcha, M. A. E. |e author | ||
| 720 | |a Ngoundou-Landji, J. |e author | ||
| 720 | |a Anumudu, C. I. |e author | ||
| 260 | |c 2009 | ||
| 520 | |a During studies on the epidemiology of malaria at a rural community, Igbo-Ora, Southwest Nigeria, genotyping of Plasmodium falciparum extracted from infective Anopheles gambiae s.l. was undertaken. Circumsporozoite (CSP) ELISA was used on crushes from head-thorax for DNA extraction and PCR amplification for the determination of P. falciparum genotypes on merozoite surface protein-I and 2 (MSP-I & 2). Of the 65 infective anophelines, P. falciparum genotypes were positively identified in 41. Mono-infections constituted 73.4% of all infections; the dominant mono-infections on MSP-1 and MSP-2 were MAD20 (18) and ICI (09) respectively; the rare RO33 (01) was recorded. Double infections were 20.20% (09) with both markers, while only one triple infection was observed on MSP-1. An anopheles was found with two double infections, one on each of the two blocks. Eight of the 12 multiple infections were on MSP-1, five on both MSP-1 and MSP-2. In addition to the multiplicity of proteins in these vectors, size polymorphism was observed in alleles, indicating vector/parasite interactions and environmental variations. These results were compared to those from human sera. | ||
| 024 | 8 | |a 1118-1931 | |
| 024 | 8 | |a Scientia Africana 8(2), pp. 9-16 | |
| 024 | 8 | |a ui_art_noutcha_genetic_2009 | |
| 024 | 8 | |a http://ir.library.ui.edu.ng/handle/123456789/1060 | |
| 245 | 0 | 0 | |a Genetic variants of Plasmodium falciparum infective Anopheles gambiae s.l. at a rural community in southwest Nigeria |